Biological Nitrogen Removal Database

A manually curated data resource for microbial nitrogen removal


Detailed information

Microorganism

Trichoderma sp. MIAE 1095

Taxonomy

  • Phylum : Ascomycota
  • Class : Sordariomycetes
  • Order : Hypocreales
  • Family : Hypocreaceae
  • Genus : Trichoderma

Isolation Source

Agricultural soils or plant roots

Enzyme Name

copper-containing nitrite reductase 

  • Encoding Gene:NirK
  • DNA Size:432 bp
  • Nucleotide FASTA sequence: Link

  • UniProt I.D: W6JHS9

Protein Information

  • Pro_GenBank I.D: BAO48091.1

  • Length:144 aa
  • Protein FASTA_sequence: Link

Information about Article

  • Reference:Maeda et al., 2015
  • Title:N2O production, a widespread trait in fungi
  • Pubmed ID:25894103.0
  • Pubmed link: Link

  • Full research link: Link

  • Abstract:N2O is a powerful greenhouse gas contributing both to global warming and ozone depletion. While fungi have been identified as a putative source of N2O, little is known about their production of this greenhouse gas. Here we investigated the N2O-producing ability of a collection of 207 fungal isolates. Seventy strains producing N2O in pure culture were identified. They were mostly species from the order Hypocreales order-particularly Fusarium oxysporum and Trichoderma spp.-and to a lesser extent species from the orders Eurotiales, Sordariales, and Chaetosphaeriales. The N2O (15)N site preference (SP) values of the fungal strains ranged from 15.8‰ to 36.7‰, and we observed a significant taxa effect, with Penicillium strains displaying lower SP values than the other fungal genera. Inoculation of 15 N2O-producing strains into pre-sterilized arable, forest and grassland soils confirmed the ability of the strains to produce N2O in soil with a significant strain-by-soil effect. The copper-containing nitrite reductase gene (nirK) was amplified from 45 N2O-producing strains, and its genetic variability showed a strong congruence with the ITS phylogeny, indicating vertical inheritance of this trait. Taken together, this comprehensive set of findings should enhance our knowledge of fungi as a source of N2O in the environment.