Biological Nitrogen Removal Database

A manually curated data resource for microbial nitrogen removal


Detailed information

Microorganism

Nitrosomonas

Taxonomy

  • Phylum : Proteobacteria
  • Class : Betaproteobacteria
  • Order : Nitrosomonadales
  • Family : Nitrosomonadaceae
  • Genus : Nitrosomonas

Isolation Source

nan

Enzyme Name

Hydroxylamine oxydoreductase

  • Encoding Gene:hao2
  • DNA Size:2546 bp
  • Nucleotide FASTA sequence: Link

  • UniProt I.D: Q9RAH8

Protein Information

  • Pro_GenBank I.D: BAA82704.1

  • Length:570 aa
  • Protein FASTA_sequence: Link

Information about Article

  • Reference:Hirota et al., 2006
  • Title:Transcriptional analysis of the multicopy hao gene coding for hydroxylamine oxidoreductase in Nitrosomonas sp. strain ENI-11
  • Pubmed ID:16926500
  • Pubmed link: Link

  • Full research link: Link

  • Abstract:The nitrifying bacterium Nitrosomonas sp. strain ENI-11 has three copies of the gene encoding hydroxylamine oxidoreductase (hao(1), hao(2), and hao(3)) on its genome. Broad-host-range reporter plasmids containing transcriptional fusion genes between hao copies and lacZ were constructed to analyze the expression of each hydroxylamine oxidoreductase gene (hao) copy individually and quantitatively. beta-Galactosidase assays of ENI-11 harboring reporter plasmids revealed that all hao copies were transcribed in the wild-type strain. Promoter analysis of hao copies revealed that transcription of hao(3) was highest among the hao copies. Expression levels of hao(1) and hao(2) were 40% and 62% of that of hao(3) respectively. Transcription of hao(1) was negatively regulated, whereas a portion of hao(3) transcription was read through transcription from the rpsT promoter. When energy-depleted cells were incubated in the growth medium, only hao(3) expression increased. This result suggests that it is hao(3) that is responsible for recovery from energy-depleted conditions in Nitrosomonas sp. strain ENI-11.