Biological Nitrogen Removal Database

A manually curated data resource for microbial nitrogen removal


Detailed information

Microorganism

Enterobacter cloacae

Taxonomy

  • Phylum : Proteobacteria
  • Class : Gammaproteobacteria
  • Order : Enterobacterales
  • Family : Enterobacteriaceae
  • Genus : Enterobacter

Isolation Source

Domestic wastewater sediments

Enzyme Name

Hydroxylamine reductase

  • Encoding Gene:hao
  • DNA Size:993 bp
  • Nucleotide FASTA sequence: Link

  • UniProt I.D: A0A060Q3B0

Protein Information

  • Pro_GenBank I.D: BAO99274.1

  • Length:331 aa
  • Protein FASTA_sequence: Link

Information about Article

  • Reference:Padhi et al., 2017
  • Title:Aerobic and heterotrophic nitrogen removal by Enterobacter cloacae CF-S27 with efficient utilization of hydroxylamine
  • Pubmed ID:28242385
  • Pubmed link: Link

  • Full research link: Link

  • Abstract:Heterotrophic bacterium, Enterobacter cloacae CF-S27 exhibited simultaneous nitrification and aerobic denitrification in presence of high concentration of hydroxylamine. With the initial nitrogen concentration of 100mgL-1h-1, ammonium, nitrate and nitrite removal efficiencies were 81%, 99.9% and 92.8%, while the corresponding maximum removal rates reached as high as 11.6, 15.1 and 11.2mgL-1h-1 respectively. Quantitative amplification by real time PCR and enzyme assay demonstrated that hydroxylamine reductase gene (hao) is actively involved in hetrotrophic nitrification and aerobic denitrification process of Enterobacter cloacae CF-S27. PCR primers were designed targeting amplification of hao gene from diversified environmental soil DNA. The strain Enterobacter cloacae CF-S27 significantly maintained the undetectable amount of dissolved nitrogen throughout 60days of zero water exchange fish culture experiment in domestic wastewater.