Biological Nitrogen Removal Database

A manually curated data resource for microbial nitrogen removal


Detailed information

Microorganism

Neobacillus vireti LMG 21834

Taxonomy

  • Phylum : Firmicutes
  • Class : Bacilli
  • Order : Bacillales
  • Family : Bacillaceae
  • Genus : Neobacillus

Isolation Source

Agricultural soil from Anlooer Diepje brrok

Enzyme Name

Nitrite reductase [NAD(P)H] large subunit

  • Encoding Gene:nirB
  • DNA Size:nan
  • Nucleotide FASTA sequence: Link

  • UniProt I.D: W1SQW5

Protein Information

  • Pro_GenBank I.D: ETI69533.1

  • Length:804 aa
  • Protein FASTA_sequence: Link

Information about Article

  • Reference:Mania et al., 2016
  • Title:Regulation of nitrogen metabolism in the nitrate-ammonifying soil bacterium Bacillus vireti and evidence for its ability to grow using N2O as electron acceptor
  • Pubmed ID:26548448
  • Pubmed link: Link

  • Full research link: Link

  • Abstract:Bacillus vireti is a nitrate-ammonifying bacterium and a partial denitrifier, reducing NO3 (-) , NO2 (-) , NO and N2 O with NarG, NrfA, CbaA and NosZ respectively. Growth is optimized through successive use of the electron acceptors O2 and NO3 (-) , followed by NO2 (-) , NO and N2 O. Fermentation takes place simultaneously with anaerobic respiration. When grown in batch culture with 5 mM initial NO3 (-) , transcription of nrfA was high and most NO3 (-) was reduced to NH4 (+) . With 20 mM initial NO3 (-) , nrfA transcription was lower and more than 50% of the nitrate was recovered as NO, N2 O and N2 . Analysis of gene transcription patterns and corresponding gas kinetics indicated that O2 and NO2 (-) or NO are main controllers of nrfA, nirB, cbaA and nosZ transcription. This was corroborated by analyses of putative binding regions for specific transcriptional regulators. Furthermore, we demonstrate that N2 O reduction in B. vireti supports growth. The high nosZ transcription but low N2 O production seen at 5 mM NO3 (-) implies that this organism can use N2 O reductase to scavenge N2 O from other organisms in the soil, thus possibly acting as a net sink for N2 O.