Biological Nitrogen Removal Database

A manually curated data resource for microbial nitrogen removal


Detailed information

Microorganism

Uncultured bacterium

Taxonomy

  • Phylum : nan
  • Class : nan
  • Order : nan
  • Family : nan
  • Genus : nan

Isolation Source

Arable soil

Enzyme Name

nitrous oxide reductase

  • Encoding Gene:NosZ
  • DNA Size:460 bp
  • Nucleotide FASTA sequence: Link

  • UniProt I.D: nan

Protein Information

  • Pro_GenBank I.D: AAS90279.1

  • Length:153 aa
  • Protein FASTA_sequence: Link

Information about Article

  • Reference:Throback et al., 2004
  • Title:Reassessing PCR Primers Targeting nirS, nirK and nosZ Genes for Community Surveys of Denitrifying Bacteria With DGGE
  • Pubmed ID:19712290
  • Pubmed link: Link

  • Full research link: Link

  • Abstract:We re-evaluated PCR primers targeting nirS, nirK and nosZ genes for denaturing gradient gel electrophoresis as a tool to survey denitrifying community composition in environmental samples. New primers for both nirS and nosZ were combined with existing primers, while for nirK the previously published F1aCu:R3Cu set was chosen for denaturing electrophoresis. All three sets yielded amplicons smaller than 500 bp and amplified the correct fragment in all environmental samples. The denaturing gradient gel electrophoresis worked satisfactorily for nirK and nosZ, but not for nirS. This was probably due to the multiple melting domains in this particular nirS fragment. From the excised and sequenced bands, only sequences related to the target genes were detected and tree analysis showed that the selected primers acted as broad range primers for each of the three genes. By use of the new nirS primers it was demonstrated that agricultural soil harbours a substantial diversity of nirS denitrifiers.