Biological Nitrogen Removal Database

A manually curated data resource for microbial nitrogen removal


Detailed information

Microorganism

Pseudomonas sp. R-25208

Taxonomy

  • Phylum : Proteobacteria
  • Class : Gammaproteobacteria
  • Order : Pseudomonadales
  • Family : Pseudomonadaceae
  • Genus : Pseudomonas

Isolation Source

nan

Enzyme Name

nitric oxide reductase CnorB

  • Encoding Gene:cNorB
  • DNA Size:434 bp
  • Nucleotide FASTA sequence: Link

  • UniProt I.D: A1JHI4

Protein Information

  • Pro_GenBank I.D: CAK95722.1

  • Length:145 aa
  • Protein FASTA_sequence: Link

Information about Article

  • Reference:Heylen et al., 2007
  • Title:Nitric oxide reductase (norB) gene sequence analysis reveals discrepancies with nitrite reductase (nir) gene phylogeny in cultivated denitrifiers.
  • Pubmed ID:17359277
  • Pubmed link: Link

  • Full research link: Link

  • Abstract:Gene sequence analysis of cnorB and qnorB, both encoding nitric oxide reductases, was performed on pure cultures of denitrifiers, for which previously nir genes were analysed. Only 30% of the 227 denitrifying strains rendered a norB amplicon. The cnorB gene was dominant in Alphaproteobacteria, and dominantly coexisted with the nirK gene, coding for the copper-containing nitrite reductase. Both norB genes were equally present in Betaproteobacteria but no linked distributional pattern of nir and norB genes could be observed. The overall cnorB phylogeny was not congruent with the widely accepted organism phylogeny based on 16S rRNA gene sequence analysis, with strains from different bacterial classes having identical cnorB sequences. Denitrifiers and non-denitrifiers could be distinguished through qnorB gene phylogeny, without further grouping at a higher taxonomic resolution. Comparison of nir and norB phylogeny revealed that genetic linkage of both genes is not widespread among denitrifiers. Thus, independent evolution of the genes for both nitrogen oxide reductases does also occur.