Biological Nitrogen Removal Database
A manually curated data resource for microbial nitrogen removal
Biological Nitrogen Removal Database
A manually curated data resource for microbial nitrogen removal
Influent:Groundwater
Denitrification system:Sulfur-driven denitrification coupled with Permeable reactive barrier (PRB)
Denitrifying reactor:Reactive Packed-bed
Medium:Granular elemental sulfur
Culture taken from:Thiobacillus denitrificans
Organism (s) cultured:nan
Respiration:Anaerobic
Electron donor:Sulphur
Electron acceptor:Nitrate
Input NO3-N (mg/l):0.36
Nitrate removal rate (mg NO3-N/l/h):0.32
Denitrification rate (gNO3-N removed/m3/day):nan
Microorganisms identified:nan
Molecular tools:nan
Major findings:Permeable reactive barrier (PRB) is an emerging technology for groundwater remediation, which has advantages over conventional remediation means such as pump-and-treat system such as cost effectiveness and a viable technology. The researchers successfully managed to remove nitrate from groundwater using the PBR coupled with SDN.
Authors:Moon et al., 2004
Title:Use of Autotrophic Sulfur-Oxidizers to Remove Nitrate From Bank Filtrate in a Permeable Reactive Barrier System
Pubmed link:Link
Full research link:Link
Abstract:This study was conducted to evaluate the potential applicability of an in situ biological reactive barrier system to treat nitrate-contaminated bank filtrate. The reactive barrier consisted of sulfur granules as an electron donor and autotrophic sulfur-oxidizing bacteria as a biological component. Limestone was also used to provide alkalinity. The results showed that the autotrophic sulfur oxidizers were successfully colonized on the surfaces of the sulfur particles and removed nitrate from synthetic bank filtrate. The sulfur-oxidizing activity continuously increased with time and then was maintained or slightly decreased after five days of column operation. Maximum nitrate removal efficiency and sulfur oxidation rate were observed at near neutral pH. Over 90% of the initial nitrate dissolved in synthetic bank filtrate was removed in all columns tested with some nitrite accumulation. However, nitrite accumulation was observed mainly during the initial operation period, and the concentration markedly diminished with time. The nitrite concentration in effluent was less than 2 mg-N/l after 12 days of column operation. When influent nitrate concentrations were 30, 40, and 60 mg-N/l and sulfur content in column was 75%, half-order autotrophic denitrification reaction rate constants were 31.73×10?3, 33.3×10?3, and 36.4×10?3 mg1/2/l1/2min, respectively. Our data on the nitrate distribution profile along the column suggest that an appropriate wall thickness of a reactive barrier for autotrophic denitrification may be 30 cm when influent nitrate concentration is less than 60 mg-N/l.