Biological Nitrogen Removal Database
A manually curated data resource for microbial nitrogen removal
Biological Nitrogen Removal Database
A manually curated data resource for microbial nitrogen removal
Influent:Artificial groundwater
Denitrification system:nan
Denitrifying reactor:nan
Medium:Na-Alginate beads
Culture taken from:Pseudomonas butanovora
Organism (s) cultured:nan
Respiration:Anaerobic
Electron donor:Succinic acid; Ethanol; Acetic acid
Electron acceptor:Nitrate
Input NO3-N (mg/l):50
Nitrate removal rate (mg NO3-N/l/h):nan
Denitrification rate (gNO3-N removed/m3/day):1630
Microorganisms identified:nan
Molecular tools:nan
Major findings:The highest denitrification activities were detected at a hydraulic retention time of 2–3 hours and all three carbon sources used as electron donors resulted in high denitrification rates.
Authors:Kesseru et al., 2002
Title:Investigation of the denitrification activity of immobilized Pseudomonas butanovora cells in the presence of different organic substrates
Pubmed link:None
Full research link:Link
Abstract:An investigation was made of the effects of variation of the hydraulic retention time (HRT) and the concentrations of three different carbon sources (succinic acid, ethanoland acetic acid) on the denitrification activity of immobilized Pseudomonas butanovora cells. The highest denitrification activity was in all cases measured at a C : N ratio of 6 : 1 and a relatively low HRT (2.5 h). The highest denitrification rates were 1.17 kg NO3 -N m3 d1 for succinic acid, 1.63 kg NO3 -N m3 d1 for ethanoland 1.53 kg NO3 -N m3 d1 for acetic acid. At the same C : N ratios, ethanoland acetic acid proved to be better substrates for the reduction of nitrate and nitrite. The determined optimum C : N ratios were 1.7870.31, 0.9570.17, 1.7670.42 for succinic acid, ethanoland acetic acid, respectively. The optimum C : N ratios for the different substrates did not change in response to an increased flow rate. At a C : N ratio of 3 : 1 and a HRT of 1.5 h, the immobilized cells did not retain their activity. Apart from the difference in the effectivity between the electron donors, the main influence on the denitrification rate was exerted by the flow rate The results of this study demonstrated that Pseudomonas butanovora can utilize all three of these carbon sources to achieve a high rate of denitrification.