Biological Nitrogen Removal Database
A manually curated data resource for microbial nitrogen removal
Biological Nitrogen Removal Database
A manually curated data resource for microbial nitrogen removal
Influent:Groundwater
Denitrification system:Sulfur limestone autotrophic denitrification (SLAD)
Denitrifying reactor:PBR (Packed bed reactor)
Medium:Sulphur granules
Culture taken from:nan
Organism (s) cultured:nan
Respiration:Anaerobic
Electron donor:Sulphur
Electron acceptor:Nitrate
Input NO3-N (mg/l):nan
Nitrate removal rate (mg NO3-N/l/h):8.42
Denitrification rate (gNO3-N removed/m3/day):nan
Microorganisms identified:nan
Molecular tools:nan
Major findings:The method developed in this study might be useful for estimation of four Monod-type kinetic parameters in other biofilm processes.
Authors:Zeng and Zhang 2005
Title:Evaluation of kinetic parameters of a sulfur–limestone autotrophic denitrification biofilm process
Pubmed link:None
Full research link:Link
Abstract:In this study, four kinetic parameters of autotrophic denitrifiers in fixed-bed sulfur–limestone autotrophic denitrification (SLAD) columns were evaluated. The curve-matching method was used by conducting 22 non-steady-state tests for estimation of half-velocity constant, Ks and maximum specific substrate utilization rate, k. To estimate the bacteria yield coefficient, Y and the decay coefficient, kd, two short term batch tests (before and after the starvation of the autotrophic denitrifiers) were conducted using a fixed-bed SLAD column where the biofilm was fully penetrated by nitrate-N. It was found that Ks=0.398 mg NO3?–N/l, k=0.15 d?1, kd=0.09–0.12 d?1, and Y=0.85–1.11 g VSS/g NO3?–N. Our results are consistent with those obtained from SLAD biofilm processes, but different from those obtained from suspended-growth systems with thiosulfate or sulfur powders as the S source. The method developed in this study might be useful for estimation of four Monod-type kinetic parameters in other biofilm processes. However, cautions must be given when the estimated parameters are used because the measurements of the biomass and the biofilm thickness could be further improved, and the assumption of sulfur being a non-limiting substrate needs to be proved.