Biological Nitrogen Removal Database
A manually curated data resource for microbial nitrogen removal
Biological Nitrogen Removal Database
A manually curated data resource for microbial nitrogen removal
Influent:Synthetic groundwater
Denitrification system:Sulfur-driven denitrification
Denitrifying reactor:PBR (Packed bed reactor)
Medium:Sulphur granules
Culture taken from:Thiobacillus denitrificans; Chlorobium limicola
Organism (s) cultured:nan
Respiration:Anaerobic
Electron donor:Sulphur
Electron acceptor:Nitrate
Input NO3-N (mg/l):nan
Nitrate removal rate (mg NO3-N/l/h):2
Denitrification rate (gNO3-N removed/m3/day):nan
Microorganisms identified:nan
Molecular tools:nan
Major findings:The presence of heavy metal ions such as Zn and Cu inhibited the denitrification activity at more than 0.5 mg/L of concentration whereas TCE and Cr (VI) did not significantly affect the nitrate removal efficiency at all levels tested. The data generated here can be used to determine design parameters for permeable reactive barrier (PRB) system using sulfur-oxidizing autotrophic denitrification reaction in groundwater.
Authors:Moon et al., 2006
Title:Effect of Reactive Media Composition and Co-Contaminants on Sulfur-Based Autotrophic Denitrification
Pubmed link:Link
Full research link:Link
Abstract:As a part of a study developing a biological reactive barrier system to treat nitrate-contaminated groundwater, the effects of reactive media composition and co-contaminants on sulfur-oxidizing autotrophic denitrification were investigated. The size of sulfur granules affected the denitrification rates; kinetic constants of 2.883, 2.949, and 0.677 mg-N1/2/L1/2/day were obtained when the granule sizes were below 2 mm, between 2 and 5 mm, and over 5 mm, respectively. When the volume ratios of sulfur to limestone were 1:1, 2:1, 3:1, and 4:1, kinetic constants of 5.490, 3.903, 4.072, and 2.984 mg-N1/2/L1/2/day were obtained, respectively. The presence of TCE up to 20 mg/L didn't significantly affect nitrate removal efficiency. At the TCE concentration of 80 mg/L, however, nitrate removal was markedly inhibited. Also, Zn and Cu inhibited the denitrification activity at more than 0.5 mg/L of concentration whereas Cr (VI) did not significantly affect the nitrate removal efficiency at all levels tested.