Biological Nitrogen Removal Database

A manually curated data resource for microbial nitrogen removal


Groundwater Water systems


Experimental setup


Influent:Septic tank effluent

Denitrification system:Sulfur-driven denitrification (SDN) system

Denitrifying reactor:PBR (Packed bed reactor)

Medium:Sulphur granules

Culture taken from:nan

Organism (s) cultured:nan

Respiration:Aerobic

Electron donor:Sulfur-limestone

Electron acceptor:Nitrate


Experimental Information


Input NO3-N (mg/l):nan

Nitrate removal rate (mg NO3-N/l/h):nan

Denitrification rate (gNO3-N removed/m3/day):nan

Microorganisms identified:nan

Molecular tools:nan


Information about Article


Major findings:The results show that the Sulfur-Limestone method is very effective in autotrophic denitrification, however production of sulfate and hardness and presence of sulfide in effluent are limiting factors in its application.

Authors:Zhang and Shan., 1999

Title:In Situ Septic Tank Effluent Denitrification Using a Sulfur?Limestone Process

Pubmed link:None

Full research link:Link

Abstract:Septic tanks are the second largest source of groundwater nitrate contamination. In this study, the feasibility of coupling a conventional lateral field with a sulfur?limestone (S/L) layer to treat nitrate in septic tank effluent was investigated using column reactors to simulate the septic tank soil adsorption system. The effects of different hydraulic loading rates, nitrogen loading rates, depth of SIL layers, and ratio of SIL?to?gravel on reactor performance were investigated. The profiles of ammonium, nitrite, nitrate, sulfate, calcium, and other parameters along the depth of reactors were measured. Significant nitrification was observed in the sand layer, while significant denitrification was observed in the S/L layer. Sulfate and hardness were produced in accordance with stoichiometric relationships. The results demonstrate that the S/L method is very effective in denitrification, while production of sulfate and hardness and existence of sulfide in effluent may be limiting factors in its application.