Biological Nitrogen Removal Database

A manually curated data resource for microbial nitrogen removal


Groundwater Water systems


Experimental setup


Influent:Drinking water

Denitrification system:Autotrophic denitrification

Denitrifying reactor:Sulfur-based autotrophic denitrifying Membrane reactor

Medium:Polyethersulfone (PES)

Culture taken from:Denitrifying bacteria obtained from a sulfur-packed column bioreactor

Organism (s) cultured:nan

Respiration:Anaerobic

Electron donor:Elemental sulfur

Electron acceptor:Nitrate


Experimental Information


Input NO3-N (mg/l):0.24

Nitrate removal rate (mg NO3-N/l/h):nan

Denitrification rate (gNO3-N removed/m3/day):nan

Microorganisms identified:nan

Molecular tools:nan


Information about Article


Major findings:Results from the pilot scale study showed that sulfur based denitrifying MBR process was effective in nitrate removal from drinking water. The results of the study can be successfully used in the design of an efficient autotrophic denitrification MBR for drinking water treatment at fullscale.

Authors:Sahinkaya et al., 2015

Title:Sulfur-based autotrophic denitrification of drinking water using a membrane bioreactor

Pubmed link:None

Full research link:Link

Abstract:Sulfur-oxidizing autotrophic denitrification process has drawn significant attention due to its high efficiency, elimination of carbon requirement and the effluent contamination by organic compounds. In the process, nitrate and sulfur are used as electron acceptor, and electron donor, respectively. In the present study, a novel sulfur based autotrophic denitrification process utilizing membrane bioreactor (MBR) was tested for nitrate removal from drinking water. A bench-scale MBR equipped with hydrophilic flat sheet polyethersulfone (PES) membranes (0.45 ?m) was used. Sulfur was externally added to the MBR considering the theoretical requirement. Almost complete denitrification efficiency was achieved when the influent nitrate concentrations were 25–50 mg NO3?-N/L at HRT as low as 5 h corresponding to nitrate loading rates up to 0.24 g NO3?-N/(L d). The generated sulfate concentrations were close to the theoretical values. Membrane fouling was not significant at fluxes ?20 L/(m2 h).