Biological Nitrogen Removal Database
A manually curated data resource for microbial nitrogen removal
Biological Nitrogen Removal Database
A manually curated data resource for microbial nitrogen removal
Influent:Tap water
Denitrification system:Lithotrophic denitrification
Denitrifying reactor:Fluidized-bed
Medium:Granular activated carbon
Culture taken from:Thiobacillus denitrificans
Organism (s) cultured:nan
Respiration:Anaerobic
Electron donor:Thiosulfate
Electron acceptor:Nitrate
Input NO3-N (mg/l):600
Nitrate removal rate (mg NO3-N/l/h):51.5-135.4
Denitrification rate (gNO3-N removed/m3/day):nan
Microorganisms identified:Thiobacillus denitrificans; Chryseobacterium hispalense; Sediminibacterium salmoneum; Dyella thiooxydans;
Molecular tools:PCR-DGGE
Major findings:This study reports on the first time use of the Ratkowsky model, which was successfully used to simulate the effect of temperature on the nitrate removal efficiency of Thiobacillus denitrificans dominated biofilm.
Authors:Zou et al., 2016
Title:High rate autotrophic denitrification in fluidized-bed biofilm reactors
Pubmed link:None
Full research link:Link
Abstract:High rate, high efficiency thiosulfate-driven autotrophic denitrification and denitritation with Thiobacillus denitrificans dominated biofilms were achieved in fluidized-bed reactors (FBRs) operated at 20.0±2.0 and 30.0±0.2°C. Complete nitrate removal was obtained even at nitrate loading rate and hydraulic retention time (HRT) of 600 mg L-1 h -1 and 10 min, respectively. Further decrease of HRT to 5 min resulted in 50% of nitrate removal efficiency. Nitrite did not accumulate when nitrate was used as electron acceptor unless HRT was decreased to 5 min. Effluent pH remained at 5.8 during denitrification. When nitrite was supplemented as the electron acceptor, denitritation effectively proceeded with the highest nitrite loading rate of 228 mg·L-1·h-1. Similar denitrification and denitritation performances were obtained at 20.0±2.0 and 30.0±0.2°C. Batch assays conducted at temperature range from 1 to 46°C, however, showed a significant impact of temperature on autotrophic denitrification. Ratkowsky model was used to estimate the minimum, optimal and maximum growth temperatures of Thiobacillus denitrificans dominated culture that were below 1, 26.6 and 50.8°C, respectively.