Biological Nitrogen Removal Database

A manually curated data resource for microbial nitrogen removal


Groundwater Water systems


Experimental setup


Influent:Groundwater

Denitrification system:Chemoautotrophic hydrogenotrophic denitrification

Denitrifying reactor:Packed-bed reactor

Medium:Gravel

Culture taken from:Activated sludge

Organism (s) cultured:nan

Respiration:Anoxic

Electron donor:Hydrogen; Carbon dioxide

Electron acceptor:Nitrate


Experimental Information


Input NO3-N (mg/l):1.44

Nitrate removal rate (mg NO3-N/l/h):nan

Denitrification rate (gNO3-N removed/m3/day):2000

Microorganisms identified:nan

Molecular tools:nan


Information about Article


Major findings:Hydrogenotrophic denitrification is economically viable for potable water treatment and use of an inexpensive support media as gravel, as well as the use of solar-electrolysis hydrogen production system further enhances this notion.

Authors:Karanasios et al., 2011

Title:Potable water hydrogenotrophic denitrification in packed-bed bioreactors coupled with a solar-electrolysis hydrogen production system

Pubmed link:None

Full research link:Link

Abstract:The aim of this study was to investigate the performance of bench-scale packed-bed reactors for hydrogenotrophic denitrification with hydrogen produced from electrolysis of water and electric energy provided by a solar cell. Two configurations were used, a single filter and a triple-column reactor, with gravel of different sizes as support media. The effect of hydrogen and carbon dioxide supply on the performance of the two systems of bioreactors under continuous operation was examined. The multi-filter system achieved high performances as it could safely treat polluted water with a low hydrogen and carbon dioxide consumption. A denitrification rate of 2 kg/m3 d was acheived for nitrate nitrogen and hydraulic loading of 1.44 g NO3 – -N/d and 11.5 m3 /m2 d, respectively. Also, a mathematical model was developed by using growth kinetics expressions for four-nutrient limitation (nitrate, nitrite, hydrogen and carbon dioxide) with inhibition by nitrate. The proposed model is capable of describing accurately enough, hydrogenotrophic denitrification under continuous operation.