Biological Nitrogen Removal Database
A manually curated data resource for microbial nitrogen removal
Biological Nitrogen Removal Database
A manually curated data resource for microbial nitrogen removal
Influent:Groundwater
Denitrification system:Chemoautotrophic hydrogenotrophic denitrification
Denitrifying reactor:Continuous flow fixed film reactor
Medium:Suspended culture
Culture taken from:Activated sludge
Organism (s) cultured:nan
Respiration:Anoxic
Electron donor:Hydrogen
Electron acceptor:Nitrate
Input NO3-N (mg/l):nan
Nitrate removal rate (mg NO3-N/l/h):nan
Denitrification rate (gNO3-N removed/m3/day):2.7–5.3
Microorganisms identified:Novel hydrogenotrophic denitrifiers
Molecular tools:PCR, 16S rRNA
Major findings:High nitrate removal rates were recorded and the microporous membrane used in this study served as an effective barrier for preventing microbial contamination of the product water. Novel hydrogenotrophic denitrifers were identified that need to be characterized.
Authors:Mansell and Schroeder., 2002
Title:Hydrogenotrophic denitrification in a microporous membrane bioreactor.
Pubmed link:Link
Full research link:Link
Abstract:Hydrogenotrophic denitrification of nitrate contaminated groundwater in a bench-scale microporous membrane bioreactor has been investigated. To prevent microbial contamination of the effluent from the reactor the nitrate-laden water treated was separated from the denitrifying culture with a 0.02 microm pore diameter membrane. Equal pressure was maintained across the membrane and nitrate was removed by molecular diffusion through the membrane and into the denitrifying culture. The system was operated with a hydrogenotrophic denitrification culture to circumvent the addition of an organic substrate to the water. Removal efficiencies ranging from 96% to 92% were achieved at influent concentrations ranging from 20 to 40 mg/L NO3(-)-N. The flux values achieved in this study were 2.7-5.3 g NO3-N m 2d(-1). The microporous membrane served as an effective barrier for preventing microbial contamination of the product water as evidenced by the effluent heterotrophic plate count of 9 (+/- 3.5) CFU/mL. The hydrogenotrophic culture was analyzed using available 16S and 23S rRNA-targeted oligonucleotide probes. It was determined that the enrichment process selected for organisms belonging to the beta subclass of Proteobacteria. Further analysis of the hydrogenotrophic culture indicated that the organisms may belong to the beta-3 subgroup of Proteobacteria and have yet to be identified as hydrogenotrophic denitrifiers.