Biological Nitrogen Removal Database

A manually curated data resource for microbial nitrogen removal


Groundwater Water systems


Experimental setup


Influent:Tap water

Denitrification system:Chemoautotrophic hydrogenotrophic denitrification

Denitrifying reactor:Hollow-fiber membrane biofilm reactor (HFMBR)

Medium:Polyurethane sponge matrixes

Culture taken from:nan

Organism (s) cultured:nan

Respiration:Anaerobic

Electron donor:Hydrogen

Electron acceptor:Nitrate


Experimental Information


Input NO3-N (mg/l):nan

Nitrate removal rate (mg NO3-N/l/h):nan

Denitrification rate (gNO3-N removed/m3/day):505

Microorganisms identified:nan

Molecular tools:nan


Information about Article


Major findings:The use of a hollow-fiber membrane biofilm reactor resulted in favorable utilization efficiences of hydrogen and removal of nitrate.

Authors:Lee and Rittman., 2002

Title:Applying a Novel Autohydrogenotrophic Hollow-Fiber Membrane Biofilm Reactor for Denitrification of Drinking Water

Pubmed link:Link

Full research link:Link

Abstract:We conducted a series of pseudo-steady-state experiments on a novel hollow-fiber membrane biofilm reactor used for denitrification of oligotrophic waters, such as drinking water. We applied a range of nitrate loadings and hydrogen pressures to establish under what conditions the system could attain three goodness-of-performance criteria: partial nitrate removal, minimization of hydrogen wasting, and low nitrite accumulation. The hollow-fiber membrane biofilm reactor could meet drinking-water standards for nitrate and nitrite while minimizing the amount of hydrogen wasted in the effluent when it was operated under hydrogen-limited conditions. For example, the system could achieve partial nitrate removals between 39% and 92%, effluent nitrate between 0.4 and 9.1 mg N/l, effluent nitrite less than 1 mg N/l, and effluent hydrogen below 0.1 mg H2/l. High fluxes of nitrate and hydrogen made it possible to have a short liquid retention time (42 min), compared with 1–13 h in other studies with hydrogen used as the electron donor for denitrification. The fluxes and concentrations for hydrogen, nitrate, and nitrite obtained in this study can be used as practical guidelines for system design.