Influent:Tap water
Denitrification system:Chemoautotrophic hydrogenotrophic denitrification
Denitrifying reactor:Semi-batch reactor and Continuous upflow reactors
Medium:Suspended culture
Culture taken from:Stock culture and Reservoir containing mineral medium
Organism (s) cultured:nan
Respiration:Anoxic
Electron donor:Hydrogen
Electron acceptor:Nitrate
Input NO3-N (mg/l):28.9
Nitrate removal rate (mg NO3-N/l/h):nan
Denitrification rate (gNO3-N removed/m3/day):nan
Microorganisms identified:nan
Molecular tools:nan
Major findings:Carrying our biological denitrification using hydrogen as an electron donor is an interesting prospect, it was demonstrated that the effluent from the continuous flow reactor could be recycled to produce hydrogenated water required for the denitrification process, with no adverse impact on the effluent quality, making the process economically feasible.
Authors:Sunger and Bose., 2009
Title:Autotrophic denitrification using hydrogen generated from metallic iron corrosion
Pubmed link:None
Full research link:Link
Abstract:Hydrogenotrophic denitrification was demonstrated using hydrogen generated from anoxic corrosion of metallic iron. For this purpose, a mixture of hydrogenated water and nitrate solution was used as reactor feed. A semi-batch reactor with nitrate loading of 2000 mg m?3 d?1 and hydraulic retention time (HRT) of 50 days produced effluent with nitrate concentration of 0.27 mg N L?1 (99% nitrate removal). A continuous flow reactor with nitrate loading of 28.9 mg m?3 d?1 and HRT of 15.6 days produced effluent with nitrate concentration of ?0.025 mg N L?1 (95% nitrate removal). In both cases, the concentration of nitrate degradation by-products, viz., ammonia and nitrite, were below detection limits. The rate of denitrification in the reactors was controlled by hydrogen availability, and hence to operate such reactors at higher nitrate loading rates and/or lower HRT than reported in the present study, hydrogen concentration in the hydrogenated water must be significantly increased.