Biological Nitrogen Removal Database

A manually curated data resource for microbial nitrogen removal


Groundwater Water systems


Experimental setup


Influent:Tap water

Denitrification system:Chemoautotrophic hydrogenotrophic denitrification

Denitrifying reactor:Semi-batch reactor and Continuous upflow reactors

Medium:Suspended culture

Culture taken from:Stock culture and Reservoir containing mineral medium

Organism (s) cultured:nan

Respiration:Anoxic

Electron donor:Hydrogen

Electron acceptor:Nitrate


Experimental Information


Input NO3-N (mg/l):28.9

Nitrate removal rate (mg NO3-N/l/h):nan

Denitrification rate (gNO3-N removed/m3/day):nan

Microorganisms identified:nan

Molecular tools:nan


Information about Article


Major findings:Carrying our biological denitrification using hydrogen as an electron donor is an interesting prospect, it was demonstrated that the effluent from the continuous flow reactor could be recycled to produce hydrogenated water required for the denitrification process, with no adverse impact on the effluent quality, making the process economically feasible.

Authors:Sunger and Bose., 2009

Title:Autotrophic denitrification using hydrogen generated from metallic iron corrosion

Pubmed link:None

Full research link:Link

Abstract:Hydrogenotrophic denitrification was demonstrated using hydrogen generated from anoxic corrosion of metallic iron. For this purpose, a mixture of hydrogenated water and nitrate solution was used as reactor feed. A semi-batch reactor with nitrate loading of 2000 mg m?3 d?1 and hydraulic retention time (HRT) of 50 days produced effluent with nitrate concentration of 0.27 mg N L?1 (99% nitrate removal). A continuous flow reactor with nitrate loading of 28.9 mg m?3 d?1 and HRT of 15.6 days produced effluent with nitrate concentration of ?0.025 mg N L?1 (95% nitrate removal). In both cases, the concentration of nitrate degradation by-products, viz., ammonia and nitrite, were below detection limits. The rate of denitrification in the reactors was controlled by hydrogen availability, and hence to operate such reactors at higher nitrate loading rates and/or lower HRT than reported in the present study, hydrogen concentration in the hydrogenated water must be significantly increased.