Biological Nitrogen Removal Database
A manually curated data resource for microbial nitrogen removal
Biological Nitrogen Removal Database
A manually curated data resource for microbial nitrogen removal
Influent:Surface water and Groundwater
Denitrification system:Autotrophic denitrification
Denitrifying reactor:Fluidized-bed MBR
Medium:nan
Culture taken from:Enriched sulfur- and Fe0 -oxidizing autotrophic denitrifying culture
Organism (s) cultured:nan
Respiration:Anaerobic
Electron donor:Sulfur; Iron
Electron acceptor:Nitrate
Input NO3-N (mg/l):nan
Nitrate removal rate (mg NO3-N/l/h):1.22
Denitrification rate (gNO3-N removed/m3/day):nan
Microorganisms identified:RT-PCR; 16S rRNA analysis
Molecular tools:At phylum level the culture was dominated by Proteobacteria, Acidobacteria, Firmicutes, Chloroflexi and Bacteroidetes.
Major findings:Autotrophic denitrification process was carried out simultaneously by sulfur and iron based autotrophic denitrifiers in the integrated system. This integrated process is particularly beneficial in that it eliminates excess sulfate generation and alkalinity requirement when reducing NO3 to N2 effectively.
Authors:Zhang et a., 2019
Title:Integrated sulfur- and iron-based autotrophic denitrification process and microbial profiling in an anoxic fluidized-bed membrane bioreactor
Pubmed link:Link
Full research link:Link
Abstract:The integrated sulfur- and Fe0-based autotrophic denitrification process in an anoxic fluidized-bed membrane bioreactor (AnFB-MBR) was developed for the nitrate-contaminated water treatment in order to control sulfate generation and avoid alkalinity supplement. The nitrate removal rate of the AnFB-MBR reached 1.22?g NO3?-N L?1d?1 with NO3?–N ranging 40–200?mg?L?1 at hydraulic retention times of 1.0–5.0?h. The denitrification in the integrated system was simultaneously carried out by sulfur- and Fe0-oxidizing autotrophic denitrifiers. The effluent sulfate generation was decreased by 29.3–70.3% and 31.2–50.9% due to the functional role of Fe0-based denitrification in the integrated system. Alkalinity produced by Fe0-oxidizing autotrophic denitrification could compensate for the alkalinity consumption by sulfur-based autotrophic denitrification. The sulfur- and Fe0-oxidizing autotrophic denitrifying bacterial consortium was composed mainly of bacteria from Thiobacillus, Sulfurimonas, and Geothrix genera. The integrated modes leads to a harmonious co-existence of sulfur- and Fe0-oxidizing denitrifying microbes, which may make a difference to the functional performance of the bioreactor. Overall, the integrated sulfur- and Fe0-based autotrophic denitrification could overcome the shortcomings of excess sulfate generation and external alkalinity supplementation compared to the sole sulfur-based autotrophic denitrification, indicating further potential for the technology in practice.