Biological Nitrogen Removal Database

A manually curated data resource for microbial nitrogen removal


Groundwater Water systems


Experimental setup


Influent:Groundwater

Denitrification system:Sulfur-driven denitrification

Denitrifying reactor:Rotating disks with membrane

Medium:Ultra filtration membrane

Culture taken from:Activated sludge

Organism (s) cultured:nan

Respiration:Aerobic

Electron donor:Sulphur

Electron acceptor:Nitrate


Experimental Information


Input NO3-N (mg/l):0.7

Nitrate removal rate (mg NO3-N/l/h):0.7

Denitrification rate (gNO3-N removed/m3/day):nan

Microorganisms identified:nan

Molecular tools:nan


Information about Article


Major findings:Successful novel method for the removal of nitrate from groundwater by coupling sulphur driven denitrification and separation membrane.

Authors:Kimura et al., 2002

Title:Nitrate Removal by a Combination of Elemental Sulfur-Based Denitrification and Membrane Filtration

Pubmed link:Link

Full research link:Link

Abstract:In this paper, a new method for removal of nitrate from groundwater, in which elemental sulfur-based denitrification (autotrophic denitrification) and membrane separation are combined, is proposed. By using a membrane, autotrophic denitrifiers, whose growth rate is considerably low, can be kept at a high concentration. The performance of the proposed process was examined through a long-term experiment in the laboratory using synthetic feed water. A rotating membrane disk module equipped with UF membrane (750,000 Da) was used in this study. Complete removal of nitrate (25 mg N/L) was achieved under the conditions of a biomass concentration of about 1000 mg protein/L and HRT of 160 min. Dissolved oxygen concentration and sulfur/biomass ratio in the membrane chamber were found to be the key factors in maintenance of high-process performance. Deterioration in membrane permeability was insignificant. It was found that membrane filtration could be continued with a water flux of 0.5 m3/m2/day for about 100 days without any chemical membrane cleaning. The proposed process, however, caused a slight increase in assimilable organic carbon. Sulfide was not detected in the denitrified water.