Biological Nitrogen Removal Database

A manually curated data resource for microbial nitrogen removal

Groundwater Water systems

Experimental setup


Denitrification system:Sulfur-driven denitrification

Denitrifying reactor:Up-flow packed-bed

Medium:Sulphur granules

Culture taken from:Thiomicrospira sp. CVO taken from oil reservior brine (Coleville enrichment)

Organism (s) cultured:nan


Electron donor:Sulfur

Electron acceptor:Nitrate

Experimental Information

Input NO3-N (mg/l):24.2

Nitrate removal rate (mg NO3-N/l/h):17.3

Denitrification rate (gNO3-N removed/m3/day):nan

Microorganisms identified:nan

Molecular tools:nan

Information about Article

Major findings: Sulfur based nitrate and nitrite removal rates in the continuous bioreactor were faster than those reported in the literature.

Authors:Sun and Nemati., 2012

Title:Evaluation of sulfur-based autotrophic denitrification and denitritation for biological removal of nitrate and nitrite from contaminated waters

Pubmed link:None

Full research link:Link

Abstract:Sulfur-based autotrophic denitrification and denitritation were investigated using an oil reservoir culture. In batch system nitrate up to 20 mM was reduced with concomitant sulfate production. With 20 mM nitrate, reduction of produced nitrite did not occur which was contrary to that under heterotrophic conditions. Reduction of nitrite as the sole substrate occurred even at 50 mM. When both sulfur and acetate were present, only acetate was used as the electron donor. In the continuous biofilm reactor maximum nitrate and nitrite removal rates of 17.3 and 13.2 mM/h, much higher than literature values, were achieved at residence times of 0.4 and 0.6 h, respectively. Bicarbonate functioned effectively as carbon source and alkaline, and eliminated the problems associated with lime addition. Based on these and our earlier findings the highest nitrate and nitrite removal rates are achieved under heterotrophic conditions with acetate, followed by autotrophic rates with sulfide, and then elemental sulfur.