Influent:Marine aquarium
Denitrification system:Fish-culture systems
Denitrifying reactor:Fluidized bed
Medium:Alginate beads
Culture taken from:Pseudomonas sp. JR12
Organism (s) cultured:Cichlids (Oreochromis mossambicus)
Respiration:nan
Electron donor:Immobilized starch
Electron acceptor:Nitrate
Input NO3-N (mg/l):nan
Nitrate removal rate (mg NO3-N/l/h):nan
Denitrification rate (gNO3-N removed/m3/day):62
Microorganisms identified:nan
Molecular tools:nan
Major findings:Alginate beads containing an immobilized denitrifying bacterium and immobilized starch can be used for removal of nitrate from water systems.
Authors:Tal et al., 2003
Title:Nitrate Removal in Aquariums by Immobilized Pseudomonas.
Pubmed link:Link
Full research link:Link
Abstract:Biological denitrification of nitrate to nitrogen gas was examined in a freshwater and a marine aquarium. Nitrate removal in the aquarium water was accomplished with denitrifiers immobilized in a freeze?dried, alginate?starch matrix. Starch served as a bacterial carbon source and cellular matrix?strengthening filler. Freeze?dried beads were placed in canisters through which nitrate?rich aquarium water was recirculated. The freshwater aquarium (100 L) contained goldfish (Carassius auratus ) at a total biomass of 390 g, whereas cichlids (Oreochromis mossambicus ) were kept at a similar stocking density in the marine aquarium. Denitrification resulted in low ambient nitrate concentrations in both aquariums. The specific nitrate removal rate of the freshwater beads was significantly higher (50 ?g of NO3?N/bead/day) than that of seawater beads (5 ?g of NO3?N/bead/day). Differences in ambient nitrate concentrations between both aquariums and diffusion limitation of nitrate to the active denitrification sites within the beads might explain these observed differences.