Biological Nitrogen Removal Database
A manually curated data resource for microbial nitrogen removal
Biological Nitrogen Removal Database
A manually curated data resource for microbial nitrogen removal
Influent:Seawater
Denitrification system:Recirculating aquaculture systems Heterotrophic Denitrification
Denitrifying reactor:Packed bed
Medium:Stationary filament biofilter substrate (BIOPOLYMA™)
Culture taken from:Bacillus sp.
Organism (s) cultured:Black tiger shrimp (Penaeus monodon)
Respiration:Aerobic
Electron donor:Ethanol/methanol
Electron acceptor:Nitrate
Input NO3-N (mg/l):165
Nitrate removal rate (mg NO3-N/l/h):nan
Denitrification rate (gNO3-N removed/m3/day):158
Microorganisms identified:nan
Molecular tools:nan
Major findings:The denitrification system was modified (carbon source, substrate and hydraulic residence time) which enhanced the removal of nitrate and the system's pH was stabilized improving the water quality. The shrimp grew quite well
Authors:Menasveta et al., 2001
Title:Design and function of a closed, recirculating seawater system with denitrification for the culture of black tiger shrimp broodstock.
Pubmed link:None
Full research link:Link
Abstract:A closed, recirculating seawater system with a denitrification process was designed for the culture of black tiger shrimp broodstock. The system comprised a circular rearing tank (9 m3 volume), a nitrifying biofilter (6 m3 volume) and denitrification process. The denitrification process comprised a deoxygenation column, a bacterial substrate column (143 L volume) and a re-aeration column connected to the biofilter. The experimental period was 81 weeks, consisting of 3 sequential trials using different substrates, bacterial inoculates and carbon sources: Trial 1- porous plastic balls for substrate, mangrove soil for inoculant and ethanol for the carbon source; Trial 2- crushed oyster shell for substrate, a strain of laboratory cultured bacteria for inoculant and ethanol for the carbon source; and Trial 3- crushed oyster shell for the substrate, no inoculant and methanol for the carbon source. The nitrifying biofilter controlled ammonium-N and nitrite-N within acceptable ranges (<0.5 and <0.2 mg L?1, respectively). The nitrate-N, however, became elevated gradually during trial 1 (<50 mg L?1). During trial 2, modification of bacterial substrate and the inoculation with denitrifying bacteria reduced nitrate-N in the denitrification column and the rearing tank (p<0.05). Changing the carbon source to methanol and increasing the hydraulic residence time in trial 3 resulted in a significant reduction (P<0.05) in nitrate-N (from >160 to <25 mg l?1) without the need for bacterial inoculation.