Biological Nitrogen Removal Database

A manually curated data resource for microbial nitrogen removal

Marine systems

Experimental setup


Denitrification system:Recirculating aquaculture systems Heterotrophic Denitrification

Denitrifying reactor:Packed bed

Medium:Fiber type plastic

Culture taken from:Alcaligenes sp.

Organism (s) cultured:Japanese Flounder (Paralichthys olivaceus)


Electron donor:Glucose

Electron acceptor:Nitrate

Experimental Information

Input NO3-N (mg/l):52-310

Nitrate removal rate (mg NO3-N/l/h):nan

Denitrification rate (gNO3-N removed/m3/day):41

Microorganisms identified:nan

Molecular tools:nan

Information about Article

Major findings:The closed seawater recirculation system coupled with a denitrification tank is a cost-effective technique that helped maintain a good water quality that enabled the culturing of Japanese Flounder.

Authors:Honda et al., 1993

Title:High Density Rearing of Japanese Flounder, Paralichthys olivaceus with a Closed Seawater Recirculation System Equipped with a Denitrification Unit.

Pubmed link:None

Full research link:Link

Abstract:Rearing experiment of Japanese flounder was carried out under high density condition using a 2.25 m3 of closed seawater recirculation system equipped with a denitrification unit. After 10 months of rearing, fish grew to 501 g of mean body weight (initial: 9g). The total fish weight in the system became 97.6 kg, and the density per unit water volume in the system reached 43.4 kg/m3. Total amount of seawater used was 2.91 m3, then the product per unit volume of seawater used was 33.5 kg/m3. The aerobic filter of the system operated satisfactorily and both ammonia and nitrite concentrations of rearing water were maintained at less than 1 mg-N/l almost through the experimental period. The denitrification unit worked efficiently and nitrate concentration decreased from 314.6 mg-N/l to 51.9 mg-N/l duaring the experimental period. These results demonstrate that intensive culture of Japanese flounder under high density condition without wasteful use of seawater for rearing is possible by using the closed seawater recirculation system equipped with the denitrification unit.