Biological Nitrogen Removal Database
A manually curated data resource for microbial nitrogen removal
Biological Nitrogen Removal Database
A manually curated data resource for microbial nitrogen removal
Influent:Marine water
Denitrification system:Marine Recirculating aquaculture systems (RAS)
Denitrifying reactor:nan
Medium:PHBV granules
Culture taken from:Marinobacter hydrocarbonoclasticus
Organism (s) cultured:nan
Respiration:Aerobic
Electron donor:PHBV
Electron acceptor:Nitrate
Input NO3-N (mg/l):nan
Nitrate removal rate (mg NO3-N/l/h):nan
Denitrification rate (gNO3-N removed/m3/day):nan
Microorganisms identified:Marinobacter hydrocarbonoclasticus RAD-2
Molecular tools:16S rRNA gene
Major findings:Marinobacter hydrocarbonoclasticus RAD-2, was isolated from a long-term PHBV-supported denitrification reactor that operated under alternate aerobic/anoxic conditions for marine RAS-effluent treatment. Have the ability to utilize of various biodegradable polymers as sole carbon
Authors:Kong et al., 2018
Title:Denitrification-Potential Evaluation and Nitrate-Removal-Pathway Analysis of Aerobic Denitrifier Strain Marinobacter hydrocarbonoclasticus RAD-2.
Pubmed link:None
Full research link:Link
Abstract:An aerobic denitrifier was isolated from a long-term poly (3-hydroxybutyrate-co-3-hydroxyvalerate) PHBV-supported denitrification reactor that operated under alternate aerobic/anoxic conditions. The strain was identified as Marinobacter hydrocarbonoclasticus RAD-2 based on 16S rRNA-sequence phylogenetic analysis. Morphology was observed by scanning electron microscopy (SEM), and phylogenetic characteristics were analyzed with the API 20NE test. Strain RAD-2 showed efficient aerobic denitrification ability when using NO3?-N or NO2?-N as its only nitrogen source, while heterotrophic nitrification was not detected. The average NO3?-N and NO2?-N removal rates were 6.47 mg/(L·h)and 6.32 mg/(L·h), respectively. Single-factor experiments indicated that a 5:10 C/N ratio, 25–40 °C temperature, and 100–150 rpm rotation speed were the optimal conditions for aerobic denitrification. Furthermore, the denitrifying gene napA had the highest expression on a transcriptional level, followed by the denitrifying genes nirS and nosZ. The norB gene was found to have significantly low expression during the experiment. Overall, great aerobic denitrification ability makes the RAD-2 strain a potential alternative in enhancing nitrate management for marine recirculating aquaculture system (RAS) practices. View Full-Text