Biological Nitrogen Removal Database
A manually curated data resource for microbial nitrogen removal
Biological Nitrogen Removal Database
A manually curated data resource for microbial nitrogen removal
Influent:Domestic wastewater treatment plant
Denitrification system:Sulfur-driven autotrophic denitrification
Denitrifying reactor:SND Continuous flow system
Medium:nan
Culture taken from:Sludge
Organism (s) cultured:nan
Respiration:Anaerobic
Electron donor:Sulfide
Electron acceptor:Nitrate or Nitrite
Input NO3-N (mg/l):nan
Nitrate removal rate (mg NO3-N/l/h):nan
Denitrification rate (gNO3-N removed/m3/day):nan
Microorganisms identified:Thermomonas; Stenotrophomonas; Thiobacillus; Thauera; Sulfurifustis; Sulfurimonas
Molecular tools:16S amplicon sequencing; qPCR; RT-qPCR
Major findings:The presence of a denitrifying community is a essential for autotrophic denitrification using sulphur-based compounds at acidic conditions.
Authors:Huang et al., 2019
Title:Performance of sulfur-based autotrophic denitrification and denitrifiers for wastewater treatment under acidic conditions
Pubmed link:None
Full research link:Link
Abstract:Autotrophic denitrification under acidic conditions using sulfide (S2?), elemental sulfur (S0), and thiosulfate (S2O32?) as electron donors are evaluated. Results from batch and column experiments show that when different S species were supplied, different pH conditions and denitrifier communities were required for denitrification to occur. Nitrate and nitrite were removed via autotrophic denitrification at pH ranging from 4 to 8, when S2? or S2O32? was the electron donor, while with S0 denitrification was only observed at pH?>?6. When S2? was used as electron donor, it was converted to S0, and S0 was not used while S2? was available. When addition of S2? was discontinued, or S2? depleted, S0 that had accumulated was used as electron donor for denitrification. These findings demonstrate that sulfur-based autotrophic denitrification can proceed under acidic conditions, but that the addition of appropriate S species and the presence of an effective denitrifier community are required.