Biological Nitrogen Removal Database

A manually curated data resource for microbial nitrogen removal


Water Treatment Plant


Experimental setup


Influent:Wastewater

Denitrification system:Litho-organotrophic denitrification

Denitrifying reactor:Inverse fluidized-bed

Medium:Polyethylene

Culture taken from:Denitrifying sludge

Organism (s) cultured:nan

Respiration:Anaerobic

Electron donor:Sulphide

Electron acceptor:Nitrate


Experimental Information


Input NO3-N (mg/l):0.1

Nitrate removal rate (mg NO3-N/l/h):0.1

Denitrification rate (gNO3-N removed/m3/day):nan

Microorganisms identified:nan

Molecular tools:nan


Information about Article


Major findings:Simultaneous removal of sulfur, nitrogen and carbon compounds from wastewaters was reported using an inverse fluidized bed reactor and the major end products are S0, N2 and CO2.

Authors:Beristain?Cardoso et al., 2008

Title:Simultaneous sulfide and acetate oxidation under denitrifying conditions using an inverse fluidized bed reactor

Pubmed link:None

Full research link:Link

Abstract:Simultaneous removal of sulfur, nitrogen and carbon compounds from wastewaters is a commercially important biological process. The objective was to evaluate the influence of the CH3COO?/NO3? molar ratio on the sulfide oxidation process using an inverse fluidized bed reactor (IFBR). Three molar ratios of CH3COO?/NO3? (0.85, 0.72 and 0.62) with a constant S2?/NO3? molar ratio of 0.13 were evaluated. At a CH3COO?/NO3? molar ratio of 0.85, the nitrate, acetate and sulfide removal efficiencies were approximately 100%. The N2 yield (g N2 g?1 NO3??N consumed) was 0.81. Acetate was mineralized, resulting in a yield of 0.65 g inorganic?C g?1 CH3COO??C consumed. Sulfide was partially oxidized to S0, and 71% of the S2? consumed was recovered as elemental sulfur by a settler installed in the IFBR. At a CH3COO?/NO3? molar ratio of 0.72, the efficiencies of nitrate, acetate and sulfide consumption were of 100%, with N2 and inorganic?C yields of 0.84 and 0.69, respectively. The sulfide was recovered as sulfate instead of S0, with a yield of 0.92 g SO42??S g?1 S2? consumed. The CH3COO?/NO3? molar ratio was shown to be an important parameter that can be used to control the fate of sulfide oxidation to either S0 or sulfate. In this study, the potential of denitrification for the simultaneous removal of organic matter, sulfide and nitrate from wastewaters was demonstrated, obtaining CO2, S0 and N2 as the major end products.