Biological Nitrogen Removal Database

A manually curated data resource for microbial nitrogen removal


Water Treatment Plant


Experimental setup


Influent:Sulfide-laden organic wastewater

Denitrification system:Simultaneous Desulfurization and Denitrification (SDD)

Denitrifying reactor:Anaerobic attached growth

Medium:Sponge cubes

Culture taken from:Waste water plant sludge

Organism (s) cultured:nan

Respiration:Anaerobic

Electron donor:Sulphide

Electron acceptor:Nitrate


Experimental Information


Input NO3-N (mg/l):2.3

Nitrate removal rate (mg NO3-N/l/h):2.3

Denitrification rate (gNO3-N removed/m3/day):nan

Microorganisms identified:nan

Molecular tools:nan


Information about Article


Major findings:Simultaneous autotrophic and heterotrophic desulfurization–denitrification process resulted in the elimination of also sulfide and organic carbon with the presence of nitrate and nitrite.

Authors:Li et al., 2009

Title:Sulfide Removal by Simultaneous Autotrophic and Heterotrophic Desulfurization-Denitrification Process

Pubmed link:Link

Full research link:Link

Abstract:An anaerobic attached-growth bioreactor (AAGBR) of 3.52 L was operated for 510 days to treat sulfide-laden organic wastewater where nitrate and nitrite were introduced as electron acceptors. When the influent sulfide was kept at 200mg S(2-)-S/L and organic carbon was increased from 20 to 33.6 mg C/L, and the hydraulic retention time decreased from 41.4 to 2.67 h, the removal rates of sulfide and organic carbon reached 99.9% and 91.8% at the loading rates of 1800 mg S(2-)-S/(Ld) and 302.4 mg C/(Ld), respectively. Simultaneously, the introduced electron acceptors of nitrate and nitrite were, respectively, removed by 99.9% and 99.9% at the loading rates of 472.5 mg NO(3)(-)-N/(Ld) and 180 mg NO(2)(-)-N/(Ld). Inside the AAGBR, both autotrophic and heterotrophic denitrification processes were noted to take place. When the influent organic carbon was increased from 20 to 33.6 mg C/L, the nitrate and nitrite consumed for heterotrophic denitrification accounted for 27.3% and 48.5%, respectively. This simultaneous autotrophic and heterotrophic desulfurization-denitrification process has provided a demonstration of the possibility to eliminate sulfide and organic carbon with the presence of nitrate and nitrite