Biological Nitrogen Removal Database

A manually curated data resource for microbial nitrogen removal


Water Treatment Plant


Experimental setup


Influent:Industrial wastewater

Denitrification system:Heterotrophic denitrification coupled with a membrane

Denitrifying reactor:Anoxic–oxic membrane

Medium:Polyvinylidene fluoride

Culture taken from:Anaerobic sludge

Organism (s) cultured:nan

Respiration:Aerobic

Electron donor:Acetate

Electron acceptor:Nitrate


Experimental Information


Input NO3-N (mg/l):8

Nitrate removal rate (mg NO3-N/l/h):8

Denitrification rate (gNO3-N removed/m3/day):nan

Microorganisms identified:nan

Molecular tools:nan


Information about Article


Major findings:The study developed a modified anoxic/oxic-membrane bioreactor which was used for the treatment of a high strength nitrate waste. The modified bioreactor could remove the residual COD, excessive biomass and soluble microbial products generated in the denitrification process successfully.

Authors:Shen et al., 2009

Title:Biological denitrification of high-nitrate wastewater in a modified anoxic/oxic-membrane bioreactor (A/O-MBR). 

Pubmed link:Link

Full research link:Link

Abstract:A modified anoxic/oxic-membrane bioreactor has been applied to the denitrification of a high strength nitrate waste (about 3600 mg/L nitrate-N) generated from an initiating explosive factory. Nitrate removal efficiency and nitrite accumulation in the treated water were investigated under various conditions set by several factors including the type of carbon source used, ratios of carbon to nitrogen, pH and hydraulic retention times (HRTs). The results of the preliminary experiments, which were carried out in parallel CSTR systems, demonstrated that sodium acetate had shown the best performance as the external carbon source. The optimal reaction parameters in the anoxic/oxic-membrane bioreactor were pH 7.5–8.5, C/N 1.56 and HRT 30 h, with over 99.9% of nitrate removed and without accumulation of nitrite. Explicitly high average-specific denitrification rate of 324 mg NO3?-N/g VSS/h could be attained under these conditions. The aerobic process and membrane module used subsequently could remove the residual COD, excessive biomass and soluble microbial products generated during the denitrification process.