Biological Nitrogen Removal Database
A manually curated data resource for microbial nitrogen removal
Biological Nitrogen Removal Database
A manually curated data resource for microbial nitrogen removal
Influent:Saline wastewater
Denitrification system:Sulfur-driven denitrification (SDN) system
Denitrifying reactor:PBR and CSTR (Continuous stirred tank reactor )
Medium:Sulphur granules
Culture taken from:Thiobacillus denitrificans
Organism (s) cultured:nan
Respiration:Anaerobic
Electron donor:Elemental sulphur
Electron acceptor:Nitrate
Input NO3-N (mg/l):nan
Nitrate removal rate (mg NO3-N/l/h):28.4 (packed bed) 1.2 (CSTR)
Denitrification rate (gNO3-N removed/m3/day):nan
Microorganisms identified:nan
Molecular tools:nan
Major findings:Autotrophic bacteria, Thiobacillus denitrificans was immobilized on surfaces of sulphur granules in the columns of a packed bed reactor and proved to be efficient in the removeal of nitrate from wastewater.
Authors:Zhao et al., 2004
Title:Nitrate Removal From Saline Water Using Autotrophic Denitrification by the Bacterium Thiobacillus Denitrificans MP-1
Pubmed link:Link
Full research link:Link
Abstract:Autotrophic denitrification of synthetic wastewater by Thiobacillus denitrificans MP-1 isolated from mangrove sediment was investigated in both up-flow packed-bed reactors and fermentor. More than 97.5% and 90% of the nitrate in inflow was removed after 8.8 and 161 hours at 250 and 195 mg l(-1) for the packed-bed reactor and fermentor system, respectively. The nitrate was quickly denitrified at very low flow rates (0.11 m h(-1)) for the packed-bed reactors, but as the flow rate was greater than 0.13 m h(-1), the nitrate removal rate increased as the flow rate increased. In the static fermentor system, the denitrification can be described by a secondary reaction, but at a flow rate between 1.31 to 1.49 m h(-1), the reactor performance can be described using the zero-order reaction in the packed-bed reactor. As the speed increases, the zero-order reaction translates into half-order reaction as the penetration efficiency of nitrate decreases. The mass ratios between the nitrate removed and the sulfate produced were determined to be 6.81 and 9.32 in the reactor column and fermentor, respectively. The results of this study suggest that efficient removal of high concentrations of nitrate in water or wastewater can be achieved effectively using autotrophic bacteria immobilized on surfaces of sulphur granules in packed-bed reactor.