Biological Nitrogen Removal Database

A manually curated data resource for microbial nitrogen removal


Water Treatment Plant


Experimental setup


Influent:Synthetic wastewater

Denitrification system:Sulfur-driven denitrification (SDN) system

Denitrifying reactor:PBR (Packed bed reactor)

Medium:nan

Culture taken from:Thiobacillus denitrificans

Organism (s) cultured:nan

Respiration:Anaerobic

Electron donor:Thiosulfate; Sulfide

Electron acceptor:Nitrate


Experimental Information


Input NO3-N (mg/l):nan

Nitrate removal rate (mg NO3-N/l/h):6.1

Denitrification rate (gNO3-N removed/m3/day):nan

Microorganisms identified:nan

Molecular tools:nan


Information about Article


Major findings:The bacteria could utilize thiosulfate and sulfide as an electron donor. Limestone was added to the reactors to stablize the pH.

Authors:Bisogni and Driscoll 1977

Title:Denitrification using thiosulfate and sulfide

Pubmed link:None

Full research link:Link

Abstract:Reliable autotrophic denitrification for removing nitrogen from wastewaters can be obtained with thiosulfate or sulfide, or both, as electron donors for enriched cultures of Thiobacillus denitrificans in completely mixed suspended growth systems. The consumptive ratio for T. denitrificans denitrification appears to be close to 1.35, the thermodynamically predicted value. Systems are tested under dynamic loading conditions, and overall operational stability is assessed.