Biological Nitrogen Removal Database

A manually curated data resource for microbial nitrogen removal


Water Treatment Plant


Experimental setup


Influent:Synthetic wastewater

Denitrification system:Sulfur–limestone autotrophic denitrification (SLAD) 

Denitrifying reactor:PBR (Packed bed reactor)

Medium:Granular elemental sulfur

Culture taken from:Continuous-stirred tank reactor CSTR seed

Organism (s) cultured:nan

Respiration:Anaerobic

Electron donor:Sulfur-limestone

Electron acceptor:Nitrate


Experimental Information


Input NO3-N (mg/l):nan

Nitrate removal rate (mg NO3-N/l/h):8.3

Denitrification rate (gNO3-N removed/m3/day):nan

Microorganisms identified:nan

Molecular tools:nan


Information about Article


Major findings:Detailed information on the interaction among some major variables on the design and performance of the SLAD process is lacking. This study they developed the response surface method was used by designing a rotatable central composite test scheme with 12 SLAD column tests. Their results suggest that this model can be applied in the estimation nitrate-nitrogen concentration and determine the nitrite control measures.

Authors:Zhang and Zeng., 2006

Title:Development of a Response Surface for Prediction of Nitrate Removal in Sulfur–Limestone Autotrophic Denitrification Fixed-Bed Reactors

Pubmed link:None

Full research link:Link

Abstract:Sulfur–limestone autotrophic denitrification (SLAD) processes are very efficient for treatment of ground or surface water contaminated with nitrate. However, detailed information is not available on the interaction among some major variables on the design and performance of the SLAD process. In this study, the response surface method was used by designing a rotatable central composite test scheme with 12 SLAD column tests. A polynomial linear regression model was set up to quantitatively describe the relationship of the effluent and influent nitrate–nitrogen concentration and hydraulic retention time (HRT) in the SLAD column reactors. This model may be used for estimating the effluent nitrate–nitrogen concentration when the influent nitrate–nitrogen concentration ranges between 20 and