Biological Nitrogen Removal Database
A manually curated data resource for microbial nitrogen removal
Biological Nitrogen Removal Database
A manually curated data resource for microbial nitrogen removal
Influent:Synthetic wastewater
Denitrification system:nan
Denitrifying reactor:Upflow anaerobic sludge bed (UASB)
Medium:Granular sludge
Culture taken from:Thiobacillus denitrificans
Organism (s) cultured:nan
Respiration:Anaerobic
Electron donor:Thiosulfate
Electron acceptor:Nitrate
Input NO3-N (mg/l):nan
Nitrate removal rate (mg NO3-N/l/h):62.5
Denitrification rate (gNO3-N removed/m3/day):nan
Microorganisms identified:nan
Molecular tools:nan
Major findings:Thiobacillus denitrificans was the predominant species over Thiomicrospira denitrificans in both upflow anaerobic sludge bed (UASB) reactors. The results strongly suggest that this bacterium was responsible for denitrification and sulfoxidation within the reactors.
Authors:Sánchez et al. 2008
Title:Assessment of the Addition of Thiobacillus Denitrificans and Thiomicrospira Denitrificans to Chemolithoautotrophic Denitrifying Bioreactors
Pubmed link:Link
Full research link:None
Abstract:The aim of the present study was to assess the impact of adding cultures of Thiobacillus denitrificans and Thiomicrospira denitrificans to two upflow anaerobic sludge bed (UASB) reactors: one inoculated with granular sludge and the other filled only with activated carbon (AC). The performances of the bioreactors and the changes in biomass were compared with a non-bioaugmented control UASB reactor inoculated with granular sludge. The reactors inoculated with granular sludge achieved efficiencies close to 90% in nitrate and thiosulfate removal for loading rates as high as 107 mmol-NO3 -/l per day and 68 mmol-S2O3 2-/l per day. Bioaugmentation with Tb. denitrificans and Tm. denitrificans did not enhance the efficiency compared to that achieved with non-bioaugmented granular sludge. The loading rates and efficiencies were 30-40% lower in the AC reactor. In all the reactors tested, Tb. denitrificans became the predominant species. The results strongly suggest that this bacterium was responsible for denitrification and sulfoxidation within the reactors. We additionally observed that granules partially lost their integrity during operation under chemolithoautotrophic conditions, suggesting limitations for long-term operation if bioaugmentation is applied in practice.