Biological Nitrogen Removal Database
A manually curated data resource for microbial nitrogen removal
Biological Nitrogen Removal Database
A manually curated data resource for microbial nitrogen removal
Influent:Synthetic wastewater
Denitrification system:Sulfur-driven denitrification (SDN) system
Denitrifying reactor:Immobilized cell reactor
Medium:Poly(vinyl alcohol) PVA
Culture taken from:Thiobacillus denitrificans
Organism (s) cultured:nan
Respiration:Anaerobic
Electron donor:Thiosulfate
Electron acceptor:Nitrate
Input NO3-N (mg/l):nan
Nitrate removal rate (mg NO3-N/l/h):1.83
Denitrification rate (gNO3-N removed/m3/day):nan
Microorganisms identified:nan
Molecular tools:nan
Major findings:The denitrification efficiencies of Thiobacillus denitrificans were compared in batch reactors between free cells and cells immobilized on polyvinyl alcohol (PVA) carriers, this is because T. denitrificans has enormous potential in the removal of nitrate from the environment in-terms of efficiency and overall costs. A prolonged denitrification process was observed when using the immobilized cells.
Authors:Zhang et al., 2009a
Title:Nitrate Removal by Thiobacillus Denitrificans Immobilized on Poly(vinyl Alcohol) Carriers
Pubmed link:Link
Full research link:Link
Abstract:Nitrate contamination is becoming a widespread environmental problem, and autotrophic denitrification with Thiobacillus denitrificans is a promising process considering efficiency, cost and maintenance. The denitrification efficiencies of T. denitrificans were compared in batch reactors between free cells and cells immobilized on polyvinyl alcohol (PVA) carriers made with thrice freezing/thawing and boric acid methods. The results indicated that the free cell reactor of T. denitrificans added with 10% (v/v) of PVA carrier made by thrice freezing/thawing (PVA-TFT) exhibited faster in S(2)O(3)(2-)-S consumption, SO(4)(2-) generation, and NO(3)(-)-N denitrification, with corresponding values being 165 mg (S(2)O(3)(2-)-S)/L.d, 491 mg (SO(4)(2-))/Ld, and 44 mg (NO(3)(-)-N)/Ld, which were increased by 50%, 61%, and 57% respectively compared to the control reactor with only free cells. Inhibition of denitrification by accumulated SO(4)(2-) in PVA-TFT reactor appeared at the concentration of approximately 6000 mg (SO(4)(2-))/L, and 75% of NO(3)(-)-N removal efficiency was achieved after 12d operation under the condition of initial 700 mg/L NO(3)(-)-N concentration.