Biological Nitrogen Removal Database
A manually curated data resource for microbial nitrogen removal
Biological Nitrogen Removal Database
A manually curated data resource for microbial nitrogen removal
Influent:Synthetic wastewater
Denitrification system:Lithotrophic denitrification
Denitrifying reactor:Expanded granular sludge bed
Medium:Sulfide granules
Culture taken from:Sulfurovum sp.
Organism (s) cultured:nan
Respiration:Anaerobic
Electron donor:Hydrogen sulfide; Acetate
Electron acceptor:Nitrate
Input NO3-N (mg/l):nan
Nitrate removal rate (mg NO3-N/l/h):59
Denitrification rate (gNO3-N removed/m3/day):nan
Microorganisms identified:nan
Molecular tools:nan
Major findings:The study reports of a novel two-stage scheme for granule incubation that was adopted to generate stable granules.
Authors:Chen et al., 2008
Title:Simultaneous biological removal of sulfur, nitrogen and carbon using EGSB reactor.
Pubmed link:Link
Full research link:Link
Abstract:High-rate biological conversion of sulfide and nitrate in synthetic wastewater to, respectively, elemental sulfur (S(0)) and nitrogen-containing gas (such as N(2)) was achieved in an expanded granular sludge bed (EGSB) reactor. A novel strategy was adopted to first cultivate mature granules using anaerobic sludge as seed sludge in sulfate-laden medium. The cultivated granules were then incubated in sulfide-laden medium to acclimate autotrophic denitrifiers. The incubated granules converted sulfide, nitrate, and acetate simultaneously in the same EGSB reactor to S(0), N-containing gases and CO(2) at loading rates of 3.0 kg S m(-3) d(-1), 1.45 kg N m(-3) d(-1), and 2.77 kg Ac m(-1) d(-1), respectively, and was not inhibited by sulfide concentrations up to 800 mg l(-1). Effects of the C/N ratio on granule performance were identified. The granules cultivated in the sulfide-laden medium have Pseudomonas spp. and Azoarcus sp. presenting the heterotrophs and autotrophs that co-work in the high-rate EGSB-SDD (simultaneous desulfurization and denitrification) reactor.