Biological Nitrogen Removal Database

A manually curated data resource for microbial nitrogen removal


Water Treatment Plant


Experimental setup


Influent:Refinery wastewater

Denitrification system:Autotrophic denitrification

Denitrifying reactor:Continuous stirred tank reactor CSTR

Medium:Suspended culture

Culture taken from:nan

Organism (s) cultured:nan

Respiration:Anaerobic

Electron donor: Acetate; Sulfide

Electron acceptor:Nitrate


Experimental Information


Input NO3-N (mg/l):nan

Nitrate removal rate (mg NO3-N/l/h):8.33

Denitrification rate (gNO3-N removed/m3/day):nan

Microorganisms identified:nan

Molecular tools:nan


Information about Article


Major findings:Results from the study suggests that denitrification is a feasible process for the simultaneous removal of nitrogen, carbon and sulfur from effluents of the petroleum industry.

Authors:Reyes-Avila et al., 2004

Title:Simultaneous biological removal of nitrogen, carbon and sulfur by denitrification

Pubmed link:Link

Full research link:Link

Abstract:Refinery wastewaters may contain aromatic compounds and high concentrations of sulfide and ammonium which must be removed before discharging into water bodies. In this work, biological denitrification was used to eliminate carbon, nitrogen and sulfur in an anaerobic continuous stirred tank reactor of 1.3 L and a hydraulic retention time of 2 d. Acetate and nitrate at a C/N ratio of 1.45 were fed at loading rates of 0.29 kg C/m3 d and 0.2 kg N/m3 d, respectively. Under steady-state denitrifying conditions, the carbon and nitrogen removal efficiencies were higher than 90%. Also, under these conditions, sulfide (S(2-)) was fed to the reactor at several sulfide loading rates (0.042-0.294 kg S(2-)/m3 d). The high nitrate removal efficiency of the denitrification process was maintained along the whole process, whereas the carbon removal was 65% even at sulfide loading rates of 0.294 kg S(2-)/m3 d. The sulfide removal increased up to approximately 99% via partial oxidation to insoluble elemental sulfur (S0) that accumulated inside the reactor. These results indicated that denitrification is a feasible process for the simultaneous removal of nitrogen, carbon and sulfur from effluents of the petroleum industry.