Biological Nitrogen Removal Database

A manually curated data resource for microbial nitrogen removal


Water Treatment Plant


Experimental setup


Influent:Synthetic wastewater 

Denitrification system:Lithotrophic-heterotrophic denitrification

Denitrifying reactor:Membrane bioreactor (MBR)

Medium:Sulfur; Calcium carbonate; Powder activated carbon

Culture taken from:Activated sludge

Organism (s) cultured:nan

Respiration:Anoxic–anaerobic

Electron donor:Elemental sulfur 

Electron acceptor:Nitrate


Experimental Information


Input NO3-N (mg/l):nan

Nitrate removal rate (mg NO3-N/l/h):nan

Denitrification rate (gNO3-N removed/m3/day):nan

Microorganisms identified:nan

Molecular tools:nan


Information about Article


Major findings:Sulfur-based carrier application to increased nitrogen removal effectiveness within the system. The sulfate that is produced as a by-product of sulfur-driven autotrophic denitrification was produced in low concentrations. An increase in elemental sulfur carriers resulted in an increase in nitrate removal.

Authors:Vo et al., 2018

Title:Nitrogen Removal by Sulfur-Based Carriers in a Membrane Bioreactor (MBR).

Pubmed link:Link

Full research link:Link

Abstract:Sulfur-based carriers were examined to enhance the nitrogen removal efficiency in a mixed anoxic?anaerobic-membrane bioreactor system, in which sulfur from the carrier acts as an electron donor for the conversion of nitrate to nitrogen gas through the autotrophic denitrification process. A total nitrogen removal efficiency of 63% was observed in the system with carriers, which showed an increase in the removal efficiency of around 20%, compared to the system without carriers. The results also indicated that the carriers had no adverse effect on biological treatment for the organic matter and total phosphorus. The removal efficiencies for chemical oxygen demand (COD) and total phosphorus (TP) were 98% and 37% in both systems, respectively. The generation of sulfate ions was a major disadvantage of using sulfur-based carriers, and resulted in pH drop. The ratio of sulfate in the effluent to nitrate removed in the system ranged from 0.86 to 1.97 mgSO?2-/mgNO?--N, which was lower than the theoretical value and could be regarded as due to the occurrence of simultaneous heterotrophic and autotrophic denitrification.