Biological Nitrogen Removal Database

A manually curated data resource for microbial nitrogen removal


Water Treatment Plant


Experimental setup


Influent:Synthetic wastewater

Denitrification system:Heterotrophic denitrification

Denitrifying reactor:Gas–liquid–solid fluidized bed bioreactor

Medium:Activated charcoal

Culture taken from:Activated sludge

Organism (s) cultured:nan

Respiration:Anaerobic

Electron donor:Methanol

Electron acceptor:Nitrate


Experimental Information


Input NO3-N (mg/l):104.6

Nitrate removal rate (mg NO3-N/l/h):nan

Denitrification rate (gNO3-N removed/m3/day):nan

Microorganisms identified:nan

Molecular tools:nan


Information about Article


Major findings:A three phase fluidized bed reactor was used for nitrate removal from wastewater. The optimum conditions for efficient and high removal rate of nitrate were 20–35 ?C, pH value of 6.5–7.5, HRT of 3 h, C/N of 0.95–1 and gas influx of 0.3 m3/h.

Authors:Wen et al., 2003

Title:The denitrification treatment of low C/N ratio nitrate-nitrogen wastewater in a gas–liquid–solid fluidized bed bioreactor

Pubmed link:None

Full research link:Link

Abstract:A 12 l gas–liquid–solid three-phase fluidized bed bioreactor in which biomass supported on activated charcoal was used to remove nitrate-nitrogen from wastewater and its performance was considered. The effects of temperature, pH, C/N ratio, gas flow rate and hydraulic residence time (HRT) on nitrate-nitrogen reduction were investigated and discussed. The optimum operated conditions such as temperature of 20–35 °C, pH value of 6.5–7.5, HRT of 3 h, C/N of 0.95–1 and gas influx of 0.3 m3/h were found. Under optimum operated conditions, the average removal ratios of COD and NOx-N are higher than 92% and 96%, respectively. In addition, the radial and axial positions have little influence on the local profiles of COD and NOx-N.