Influent:Wastewater
Denitrification system:Heterotrophic-Autotrophic denitrification
Denitrifying reactor:Sulfur packed bed reactor
Medium:Granular sulfur
Culture taken from:Tidal flats
Organism (s) cultured:nan
Respiration:Anaerobic
Electron donor:Methanol
Electron acceptor:Nitrate
Input NO3-N (mg/l):750
Nitrate removal rate (mg NO3-N/l/h):nan
Denitrification rate (gNO3-N removed/m3/day):nan
Microorganisms identified:nan
Molecular tools:nan
Major findings:Coupling heterotrophic denitrifcation to sulfur driven autotrophic denitrification presented an advantaged in this study because heterotrophic denitrification was a source of alkanility which enabled the system to prevent formation of sulfate and ensuring the effective removal of nitrate.
Authors:Kim and Bae., 2000
Title:Alkalinity requirements and the possibility of simultaneous heterotrophic denitrification during sulfur-utilizing autotrophic denitrification
Pubmed link:None
Full research link:Link
Abstract:Alkalinity requirement and the possibility of simultaneous heterotrophic denitrification during sulfur-utilizing autotrophic denitrification were evaluated with sulfur packed bed reactors (SPBRs). SPBR showed >99% NO3--N removal efficiency at influent NO3--N concentration of 1,500 mg/L, although 25-40% of the added NO3--N was recovered as N2O. Complete denitrification without N2O production was achieved when the influent NO3--N concentration decreased to 750 mg/L. When nitrified landfill leachate containing 602–687 mg/L of NO3--N was fed to SPBR, denitrification efficiency was greater than 98%. During leachate treatment, alkalinity consumption was 3.25–3.76 g CaCO3/g NO3--N removed. Most of denitrification activity occurred within bottom 11.5 cm of sulfur layer, meaning that effective HRT of 2.34 hours was enough for the complete denitrification at the loading rate of 2.2 kg NO3--N/m3-day. Complete denitrification was also achieved when methanol was added to nitrified leachate without alkalinity addition. In this case, alkalinity produced by heterotrophs was used for sulfur-utilizing denitrification.