Biological Nitrogen Removal Database

A manually curated data resource for microbial nitrogen removal


Water Treatment Plant


Experimental setup


Influent:Wastewater

Denitrification system:Heterotrophic-Autotrophic denitrification

Denitrifying reactor:Sulfur packed bed reactor

Medium:Granular sulfur

Culture taken from:Tidal flats

Organism (s) cultured:nan

Respiration:Anaerobic

Electron donor:Methanol

Electron acceptor:Nitrate


Experimental Information


Input NO3-N (mg/l):750

Nitrate removal rate (mg NO3-N/l/h):nan

Denitrification rate (gNO3-N removed/m3/day):nan

Microorganisms identified:nan

Molecular tools:nan


Information about Article


Major findings:Coupling heterotrophic denitrifcation to sulfur driven autotrophic denitrification presented an advantaged in this study because heterotrophic denitrification was a source of alkanility which enabled the system to prevent formation of sulfate and ensuring the effective removal of nitrate.

Authors:Kim and Bae., 2000

Title:Alkalinity requirements and the possibility of simultaneous heterotrophic denitrification during sulfur-utilizing autotrophic denitrification

Pubmed link:None

Full research link:Link

Abstract:Alkalinity requirement and the possibility of simultaneous heterotrophic denitrification during sulfur-utilizing autotrophic denitrification were evaluated with sulfur packed bed reactors (SPBRs). SPBR showed >99% NO3--N removal efficiency at influent NO3--N concentration of 1,500 mg/L, although 25-40% of the added NO3--N was recovered as N2O. Complete denitrification without N2O production was achieved when the influent NO3--N concentration decreased to 750 mg/L. When nitrified landfill leachate containing 602–687 mg/L of NO3--N was fed to SPBR, denitrification efficiency was greater than 98%. During leachate treatment, alkalinity consumption was 3.25–3.76 g CaCO3/g NO3--N removed. Most of denitrification activity occurred within bottom 11.5 cm of sulfur layer, meaning that effective HRT of 2.34 hours was enough for the complete denitrification at the loading rate of 2.2 kg NO3--N/m3-day. Complete denitrification was also achieved when methanol was added to nitrified leachate without alkalinity addition. In this case, alkalinity produced by heterotrophs was used for sulfur-utilizing denitrification.