Biological Nitrogen Removal Database

A manually curated data resource for microbial nitrogen removal


Water Treatment Plant


Experimental setup


Influent:Wastewater

Denitrification system:Autotrophic denitrification

Denitrifying reactor:Packed-bed reactor (PBR)

Medium:Sulfur granules

Culture taken from:Denitrifying activated sludge

Organism (s) cultured:nan

Respiration:Anaerobic

Electron donor:Sulfur

Electron acceptor:Nitrate


Experimental Information


Input NO3-N (mg/l):6.25

Nitrate removal rate (mg NO3-N/l/h):1.3-10.0

Denitrification rate (gNO3-N removed/m3/day):0.3

Microorganisms identified:nan

Molecular tools:nan


Information about Article


Major findings:Optimization of sulfur based denitrification in pilot scale reactors indicate that alkalinity turns to limit the process at high loading rates.

Authors:Sahinkaya et al., 2014

Title:Pilot and full scale applications of sulfur-based autotrophic denitrification process for nitrate removal from activated sludge process effluent

Pubmed link:None

Full research link:Link

Abstract:Sulfur-based autotrophic denitrification of nitrified activated sludge process effluent was studied in pilot and full scale column bioreactors. Three identical pilot scale column bioreactors packed with varying sulfur/lime-stone ratios (1/1–3/1) were setup in a local wastewater treatment plant and the performances were compared under varying loading conditions for long-term operation. Complete denitrification was obtained in all pilot bioreactors even at nitrate loading of 10 mg NO3?–N/(L.h). When the temperature decreased to 10 °C during the winter time at loading of 18 mg NO3?–N/(L.h), denitrification efficiency decreased to 60–70% and the bioreactor with S/L ratio of 1/1 gave slightly better performance. A full scale sulfur-based autotrophic denitrification process with a S/L ratio of 1/1 was set up for the denitrification of an activated sludge process effluent with a flow rate of 40 m3/d. Almost complete denitrification was attained with a nitrate loading rate of 6.25 mg NO3?–N/(L.h).