Biological Nitrogen Removal Database

A manually curated data resource for microbial nitrogen removal


Water Treatment Plant


Experimental setup


Influent:Synthetic wastewater

Denitrification system:Heterotrophic denitrification

Denitrifying reactor:Sequencing Batch Reactor (SBR)

Medium:Suspended culture

Culture taken from:Sludge

Organism (s) cultured:nan

Respiration:Anaerobic

Electron donor:Carbon Source: (CS1) is the wastewater produced in the cleaning of the reactors used in the production of sweets; (CS2) is a saccharose-rich residue from the production of soft drinks; and (CS3) is a lactic acid-rich residue from a dairy plant.

Electron acceptor:Nitrate


Experimental Information


Input NO3-N (mg/l):nan

Nitrate removal rate (mg NO3-N/l/h):nan

Denitrification rate (gNO3-N removed/m3/day):30-48

Microorganisms identified:nan

Molecular tools:nan


Information about Article


Major findings:Organic waste from a sweet factory and soft drinks factory are sugar-rich carbon sources and the disadvantage with their use is that there is an increased management cost in the reactor maintainance due to purging of the sludge. Organic waste from diary factory had a low yield coefficient and denitrification rates was high.

Authors:Fernández-Nava et al., 2010

Title:Denitrification of High Nitrate Concentration Wastewater Using Alternative Carbon Sources

Pubmed link:Link

Full research link:Link

Abstract:The use of different organic carbon sources in the denitrification of wastewater containing 2500 mg nitrates/L in a SBR was studied. Three alternative sources of carbon were tested: wastewater from a sweet factory, a residue from a soft drinks factory and a residue from a dairy plant. The first two are sugar-rich, whereas the third presents a high content in lactic acid. Maximum specific denitrification rates of between 42 and 48 mg NO(3)-N/g VSS h were obtained. The effluents were nitrate-free and very low COD concentrations were obtained in 4-6h reaction time, especially with the sugar-rich carbon sources. The values of the denitrifier net yield coefficient were higher than when using methanol (0.93-1.75 g VSS(formed)/g NO(x)-N(reduced)). The lowest value was obtained using the lactic acid-rich residue. The optimum COD/N ratios varied between 4.6 for the lactic acid-rich carbon source and 5.5-6.5 for the sugar-rich carbon sources.