Biological Nitrogen Removal Database

A manually curated data resource for microbial nitrogen removal


Water Treatment Plant


Experimental setup


Influent:Biomass from a wastewater treament plant

Denitrification system:Heterotrophic denitrification

Denitrifying reactor:Fed-batch reactor

Medium:Suspended culture

Culture taken from:Sludge

Organism (s) cultured:nan

Respiration:Anaerobic

Electron donor:Sodium acetate

Electron acceptor:Nitrate


Experimental Information


Input NO3-N (mg/l):nan

Nitrate removal rate (mg NO3-N/l/h):nan

Denitrification rate (gNO3-N removed/m3/day):19.79

Microorganisms identified:nan

Molecular tools:nan


Information about Article


Major findings:Fed-batch mode was implemented to perform denitrification studies to compare the efficiencies different carbon sources for nitrate removal.

Authors:Bilanovic et al., 1999

Title:Denitrification under high nitrate concentration and alternating anoxic conditions

Pubmed link:None

Full research link:Link

Abstract:Denitrification under high nitrate concentration was studied in a fed-batch mode. Methanol, sodium acetate and effluent from anaerobic digesters (AD) were compared as the carbon sources. An alternating oxy–anoxy regime (10 min aeration over a period of 110 min) seems to promote the establishment of denitrifying bio-flocks of good settling characteristics. The bio-flocks produced in an anoxic reactor were of inferior settling characteristics. Bio-flocks at concentrations of 4 to 5 g VSS/l denitrify efficiently on all three carbon sources tested. A maximum nitrate removal rate was found to be 0.486 g NO3?-N/(g VSS day). Nitrite accumulated initially but its concentration decreased with time and eventually reached zero when acetate or AD effluent were used as the carbon source.