Biological Nitrogen Removal Database

A manually curated data resource for microbial nitrogen removal


Water Treatment Plant


Experimental setup


Influent:Wastewater from the stainless steel industry

Denitrification system:Heterotrophic denitrification

Denitrifying reactor:Sequencing Batch Reactor (SBR)

Medium:Suspended culture

Culture taken from:Activated sludge

Organism (s) cultured:nan

Respiration:Anaerobic

Electron donor:Methanol

Electron acceptor:Nitrate


Experimental Information


Input NO3-N (mg/l):nan

Nitrate removal rate (mg NO3-N/l/h):nan

Denitrification rate (gNO3-N removed/m3/day):30.4

Microorganisms identified:nan

Molecular tools:nan


Information about Article


Major findings:The high COD concentration of methanol enables for complete denitrification in the system. Calcium presence in the wastewater has a negative impact on the denitrification process.

Authors:Fernández-Nava et al., 2008

Title:Denitrification of wastewater containing high nitrate and calcium concentrations.

Pubmed link:None

Full research link:Link

Abstract:The removal of nitrate from rinse wastewater generated in the stainless steel manufacturing process by denitrification in a sequential batch reactor (SBR) was studied. Two different inocula from wastewater treatment plants were tested. The use of an inoculum previously acclimated to high nitrate concentrations led to complete denitrification in 6 h (denitrification rate: 22.8 mg NO 3 -N=g VSS h), using methanol as carbon source for a COD/N ratio of 4 and for a content of calcium in the wastewater of 150 mg/L. Higher calcium concentrations led to a decrease in the biomass growth rate and in the denitrification rate. The optimum COD/N ratio was found to be 3.4, achieving 98% nitrate removal in 7 h at a maximum rate of 30.4 mg NO 3 -N=g VSS h and very low residual COD in the effluent.