Biological Nitrogen Removal Database

A manually curated data resource for microbial nitrogen removal


Water Treatment Plant


Experimental setup


Influent:Wastewaters of the yeasts production

Denitrification system:Heterotrophic denitrification

Denitrifying reactor:Continuous-flow stirred reactor

Medium:Suspended culture

Culture taken from:Activated sludge

Organism (s) cultured:nan

Respiration:Anaerobic–aerobic

Electron donor:Methanol

Electron acceptor:Nitrate


Experimental Information


Input NO3-N (mg/l):3.11–7.10

Nitrate removal rate (mg NO3-N/l/h):nan

Denitrification rate (gNO3-N removed/m3/day):142

Microorganisms identified:Pseudomonas and Paracoccus sp. strains

Molecular tools:nan


Information about Article


Major findings:A mixed bacterial culture was able to perform complete denitrification at high rates at a slightly acidic pH. The strains that were identified are Pseudomonas stutzeri and Paracoccus sp. being the dominant species; other identified species were Xanthomonas malthophilia, Ochrobactrum antrophi, Aeromonas salmonicida achromogenes and Staphylococcus sp.

Authors:Foglar et al., 2005

Title:High Nitrate Removal From Synthetic Wastewater With the Mixed Bacterial Culture

Pubmed link:Link

Full research link:Link

Abstract:The applicability of the mixed bacterial culture, originated from two-stage anaerobic-aerobic industrial yeasts production wastewater treatment plant for high rate denitrification processes was investigated. After acclimation to nitrate, the dominant strains were Pseudomonas and Paracoccus sp. Complete denitrification with low accumulation of nitrite-N (0.1 mg/l) was found in synthetic wastewater, obeying a zero-order reaction with respect to nitrate and a first-order reaction with respect to biomass concentration. Denitrification was then monitored in the continuous-flow stirred reactor at different hydraulic retention time, HRT (62-28 h) in order to achieve the optimal HRT. Nitrate was completely removed during following 45 days, at 25 degrees C with HRT, which we reduced from 62 to 28 h. Yet still, at 28 h HRT, high average specific denitrification rate of 142 mg NO3- -N/g VSS h was obtained.