Biological Nitrogen Removal Database

A manually curated data resource for microbial nitrogen removal


Water Treatment Plant


Experimental setup


Influent:Synthetic wastewater

Denitrification system:Chemoautotrophic denitrification-Nitrate-dependent anaerobic ferrous oxidizing (NAFO)

Denitrifying reactor:Up-flow anaerobic sludge bed reactor (UASB)

Medium:nan

Culture taken from:Anaerobic granular sludge taken from a full-scale reactor treating paper mill effluents

Organism (s) cultured:nan

Respiration:Anoxic

Electron donor:Ferrous iron

Electron acceptor:Nitrate


Experimental Information


Input NO3-N (mg/l):0.16

Nitrate removal rate (mg NO3-N/l/h):0.12

Denitrification rate (gNO3-N removed/m3/day):nan

Microorganisms identified:nan

Molecular tools:nan


Information about Article


Major findings:The results obtained in this study shows that NAFO system is viable as a nitrate removal technology.The iron compounds (ferric salts) that accumulated in the NAFO sludge tank were used for phosphorus removal from wastewater at a removal rate of 94%.

Authors:Zhang et al., 2015

Title:Performance of nitrate-dependent anaerobic ferrous oxidizing (NAFO) process: A novel prospective technology for autotrophic denitrification

Pubmed link:Link

Full research link:Link

Abstract:Nitrate-dependent anaerobic ferrous oxidizing (NAFO) is a valuable biological process, which utilizes ferrous iron to convert nitrate into nitrogen gas, removing nitrogen from wastewater. In this work, the performance of NAFO process was investigated as a nitrate removal technology. The results showed that NAFO system was feasible for autotrophic denitrification. The volumetric loading rate (VLR) and volumetric removal rate (VRR) under steady state were 0.159 ± 0.01 kg-N/(m3 d) and 0.073 ± 0.01 kg-N/(m3 d), respectively. In NAFO system, the effluent pH was suggested as an indicator which demonstrated a good correlation with nitrogen removal. The nitrate concentration was preferred to be less than 130 mg-N/L. Organic matters had little influence on NAFO performance. Abundant iron compounds were revealed to accumulate in NAFO sludge with peak value of 51.73% (wt), and they could be recycled for phosphorus removal, with capacity of 16.57 mg-P/g VS and removal rate of 94.77 ± 2.97%, respectively.