Biological Nitrogen Removal Database
A manually curated data resource for microbial nitrogen removal
Biological Nitrogen Removal Database
A manually curated data resource for microbial nitrogen removal
Influent:Synthetic wastewater
Denitrification system:Chemoautotrophic denitrification-Nitrate-dependent anaerobic ferrous oxidizing (NAFO)
Denitrifying reactor:Lab scale Sequencing Batch Reactor (SBR)
Medium:Suspended culture
Culture taken from:Anaerobic granular sludge taken from a full-scale reactor treating paper mill effluents
Organism (s) cultured:nan
Respiration:Anaerobic
Electron donor:Ferrous iron
Electron acceptor:Nitrate
Input NO3-N (mg/l):35.14
Nitrate removal rate (mg NO3-N/l/h):0.07
Denitrification rate (gNO3-N removed/m3/day):nan
Microorganisms identified:Dokdonella sp; Ignavibacterium sp.; Rhizomicrobium sp.; Sideroxydans sp.; Bacilllus sp
Molecular tools:16S rRNA
Major findings:When operating high-efficiency NAFO reactors heteretrophic denitrification sludge can serve as bioaugmentation or a new seeding sludge. The removal of nitrate and ferrous iron was twice as the normal system without HDS serving as bioaugmentation.
Authors:Wang et al., 2015
Title:Bioaugmentation of Nitrate-Dependent Anaerobic Ferrous Oxidation by Heterotrophic Denitrifying Sludge Addition: A Promising Way for Promotion of Chemoautotrophic Denitrification
Pubmed link:Link
Full research link:Link
Abstract:Nitrate-dependent anaerobic ferrous oxidation (NAFO) is a new and valuable bio-process for the treatment of wastewaters with low C/N ratio, and the NAFO process is in state of the art. The heterotrophic denitrifying sludge (HDS), possessing NAFO activity, was used as bioaugmentation to enhance NAFO efficiency. At a dosage of 6% (V/V), the removal of nitrate and ferrous was 2.4 times and 2.3 times of as primary, and the volumetric removal rate (VRR) of nitrate and ferrous was 2.4 times and 2.2 times of as primary. Tracing experiments of HDS indicated that the bioaugmentation on NAFO reactor was resulted from the NAFO activity by HDS itself. The predominant bacteria in HDS were identified as Thauera (52.5%) and Hyphomicrobium (20.0%) which were typical denitrifying bacteria and had potential ability to oxidize ferrous. In conclusion, HDS could serve as bioaugmentation or a new seeding sludge for operating high-efficiency NAFO reactors.