Biological Nitrogen Removal Database

Uncultured ammonia-oxidizing bacterium

• Phylum : nan
• Class : nan
• Order : nan
• Family : nan
• Genus : nan

Soil

Ammonia monooxygenase subunit A

• Encoding Gene:amoA
• DNA Size：492 bp
• Nucleotide FASTA sequence： Link

• UniProt I.D: nan

• Pro_GenBank I.D： nan

• Length：nan
• Protein FASTA_sequence: Link

• Reference：Liang et al., 2014
• Title：Community structure analysis of soil ammonia oxidizers during vegetation restoration in southwest China
• Pubmed ID：23897748.0
• Pubmed link： Link

• Full research link： Link

• Abstract：Soil ammonia oxidizers play a critical role in nitrogen cycling and ecological restoration. The composition and structure of soil ammonia oxidizers and their impacting factors were studied in four typical ecosystem soils, tussock (T), shrub (S), secondary forest (SF), and primary forest (PF), during vegetation restoration in the Karst region of Southwest China. The composition and structure of the ammonia-oxidizing bacteria (AOB) and ammonia-oxidizing archaea (AOA) communities were characterized by sequencing the amoA and arch-amoA genes, respectively. The diversity of soil ammonia oxidizers (except in S) and plant Shannon diversity index gradually increased with vegetation restoration, and the ammonia oxidizer communities differed significantly (p < 0.001). Amplicons of AOA from the Nitrososphaera cluster dominated all four ecosystem soils. AOB Nitrosospira cluster 3b only appeared in PF and SF soils, while Nitrosospira cluster 3a species were found in all soils. Changes in AOB paralleled the changes in soil ammonium content that occurred with vegetation restoration. Redundancy analysis showed that the distribution of dominant AOB species was linked to pH, soil urease activity, and soil C/N ratio, whereas the distribution of dominant AOA species was mainly influenced by litter nitrogen content and C/N ratio. These results suggested that the composition and structure of the AOB community were more sensitive to changes in vegetation and soil ammonium content, and may be an important indicator of nitrogen availability in Karst ecosystem soils.