Biological Nitrogen Removal Database

A manually curated data resource for microbial nitrogen removal


Detailed information

Microorganism

Uncultured fungus

Taxonomy

  • Phylum : nan
  • Class : nan
  • Order : nan
  • Family : nan
  • Genus : nan

Isolation Source

Surface-fertilized cropland soil 

Enzyme Name

copper-containing nitrite reductase 

  • Encoding Gene:NirK
  • DNA Size:483 bp
  • Nucleotide FASTA sequence: Link

  • UniProt I.D: A0A060PPL3

Protein Information

  • Pro_GenBank I.D: BAO96061.1

  • Length:161 aa
  • Protein FASTA_sequence: Link

Information about Article

  • Reference:Wei et al., 2015
  • Title:Development of PCR primers targeting fungal nirK to study fungal denitrification in the environment
  • Pubmed ID:nan
  • Pubmed link: Link

  • Full research link: Link

  • Abstract:Fungal denitrification in soils is receiving considerable attention as one of the dominant N2O production processes. However, because of the lack of a methodology to detect fungal denitrification-related genes, the diversity and ecological behavior of denitrifying fungi in soil remains unknown. Thus, we designed a primer set to detect the fungal nitrite reductase gene (nirK) and validated its sensitivity and specificity. Through clone library analyses, we identified congruence between phylogenies of the 18S rRNA gene and nirK of denitrifying fungal isolates obtained from the surface-fertilized cropland soil and showed that fungi belonging to Eurotiales, Hypocreales, and Sordariales were primarily responsible for N2O emissions in the soil.