Biological Nitrogen Removal Database

A manually curated data resource for microbial nitrogen removal


Detailed information

Microorganism

Pseudomonas stutzeri strain YG-24

Taxonomy

  • Phylum : Proteobacteria
  • Class : Gammaproteobacteria
  • Order : Pseudomonadales
  • Family : Pseudomonadaceae
  • Genus : Pseudomonas

Isolation Source

Sediment

Enzyme Name

nitrate reductase NapA

  • Encoding Gene:NapA
  • DNA Size:2505 bp
  • Nucleotide FASTA sequence: Link

  • UniProt I.D: nan

Protein Information

  • Pro_GenBank I.D: AIU34639.1

  • Length:834 aa
  • Protein FASTA_sequence: Link

Information about Article

  • Reference:Li et al., 2015
  • Title:Removal of Nitrogen by Heterotrophic Nitrification-Aerobic Denitrification of a Phosphate Accumulating Bacterium Pseudomonas Stutzeri YG-24
  • Pubmed ID:25668754.0
  • Pubmed link: Link

  • Full research link: Link

  • Abstract:Phosphate accumulating bacterium Pseudomonas stutzeri YG-24 exhibited efficient heterotrophic nitrification and aerobic denitrification ability. Single factor experiments showed that both heterotrophic nitrification and aerobic denitrification occurred with sodium citrate as carbon source and lower C/N ratio of 8. High average NH4+-N, NO2−-N and NO3−-N removal rates of 8.75, 7.51 and 7.73 mg L−1 h−1 were achieved. The application of strain YG-24 in wastewater samples resulted in TN, NH4+-N, NO2−-N, NO3−-N and P removal efficiencies of 85.28%, 88.13%, 86.15%, 70.83% and 51.21%. Sequencing and quantitative amplification by real-time PCR of napA, nirS and ppk showed that nitrogen removal pathway of strain YG-24 was achieved through heterotrophic ammonium nitrification coupled with fast nitrite denitrification (NH4+-N to NO2−-N and then to gaseous nitrogen) directly. These results demonstrated the strain as a suitable candidate to simultaneously remove both nitrogen and phosphate in wastewater treatment.