AF083948.1

Detailed information

Microorganism
Pseudomonas sp. G-179

Taxonomy
Phylum : Proteobacteria Class : Alphaproteobacteria Order : Rhizobiales Family : Rhizobiaceae Genus : nan

Isolation Source
nan

Enzyme Name
cytochrome c binding protein

Protein Information
Pro_GenBank I.D： AAC79445.1 Length：234 aa Protein FASTA_sequence: Link

Information about Article
Reference：Bedzyk et al., 1999 Title：The periplasmic nitrate reductase in Pseudomonas sp. strain G-179 catalyzes the first step of denitrification Pubmed ID：10217771.0 Pubmed link： Link Full research link： Link Abstract：Both membrane-bound and periplasmic nitrate reductases have been found in denitrifying bacteria. Yet the role of periplasmic nitrate reductase in denitrification has not been clearly defined. To analyze the function of the periplasmic nitrate reductase in Pseudomonas sp. strain G-179, the nap gene cluster was identified and found to be linked to genes involved in reduction of nitrite and nitric oxide and anaerobic heme biosynthesis. Mutation in the nap region rendered the cells incapable of growing under anaerobic conditions with nitrate as the alternative electron acceptor. No nitrate reduction activity was detected in the Nap- mutant, but that activity could be restored by complementation with the nap region. Unlike the membrane-bound nitrate reductase, the nitrate reduction activity in strain G-179 was not inhibited by a low concentration of azide. Nor could it use NADH as the electron donor to reduce nitrate or use chlorate as the alternative substrate. These results suggest that the periplasmic nitrate reductase in this strain plays a primary role in dissimilatory nitrate reduction.

Reference：Bedzyk et al., 1999
Title：The periplasmic nitrate reductase in Pseudomonas sp. strain G-179 catalyzes the first step of denitrification
Pubmed ID：10217771.0
Pubmed link： Link
Full research link： Link
Abstract：Both membrane-bound and periplasmic nitrate reductases have been found in denitrifying bacteria. Yet the role of periplasmic nitrate reductase in denitrification has not been clearly defined. To analyze the function of the periplasmic nitrate reductase in Pseudomonas sp. strain G-179, the nap gene cluster was identified and found to be linked to genes involved in reduction of nitrite and nitric oxide and anaerobic heme biosynthesis. Mutation in the nap region rendered the cells incapable of growing under anaerobic conditions with nitrate as the alternative electron acceptor. No nitrate reduction activity was detected in the Nap- mutant, but that activity could be restored by complementation with the nap region. Unlike the membrane-bound nitrate reductase, the nitrate reduction activity in strain G-179 was not inhibited by a low concentration of azide. Nor could it use NADH as the electron donor to reduce nitrate or use chlorate as the alternative substrate. These results suggest that the periplasmic nitrate reductase in this strain plays a primary role in dissimilatory nitrate reduction.