Biological Nitrogen Removal Database

Rhodobacter sphaeroides f. sp. denitrificans IL106

• Phylum : Proteobacteria
• Class : Alphaproteobacteria
• Order : Rhodobacterales
• Family : Rhodobacteraceae
• Genus : Rhodobacter

nan

soluble protein

• Encoding Gene:napD
• DNA Size：7517 bp
• Nucleotide FASTA sequence： Link

• UniProt I.D: O88156

• Pro_GenBank I.D： BAA31960.1

• Length：88 aa
• Protein FASTA_sequence: Link

• Reference：Liu et al., 1999
• Title：Involvement in denitrification of the napKEFDABC genes encoding the periplasmic nitrate reductase system in the denitrifying phototrophic bacterium Rhodobacter sphaeroides f. sp. denitrificans
• Pubmed ID：10227138.0
• Pubmed link： Link

• Full research link： Link

• Abstract：Seven genes, napKEFDABC, encoding the periplasmic nitrate reductase system were cloned from the denitrifying phototrophic bacterium Rhodobacter sphaeroides f. sp. denitrificans IL106. Two transmembrane proteins, NapK and NapE, an iron-sulfur protein NapF, a soluble protein NapD, a catalytic subunit of nitrate reductase precursor NapA, a soluble c-type diheme cytochrome precursor NapB, and a membrane-anchored c-type tetraheme cytochrome NapC were deduced as the gene products. Every mutant in which each nap gene was disrupted by Ω-cassette insertion lost nitrate reductase activity as well as the ability of cells to grow with nitrate under anaerobic-dark conditions. A transconjugant of the napD-disrupted mutant with a plasmid bearing the napKEFDABC genes recovered both nitrate reductase activity and nitrate-dependent anaerobic-dark growth of cells. Denitrification activity, which was not observed in the napD mutant, was also restored by the conjugation. These results indicate that the periplasmic nitrate reductase encoded by the napKEFDABC genes is the enzyme responsible for denitrification in this phototroph, although the presence of a membrane-bound nitrate reductase has been reported in the same strain.